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Correction to: Recombinant production of the lantibiotic nisin using Corynebacterium glutamicum in a two‑step process

The Original Article was published on 15 January 2022

Correction to: Microbial Cell Factories (2022) 21:11 https://doi.org/10.1186/s12934-022–01739-y

Following publication of the original article [1], the authors identified an error in Fig. 3d. The correct figure (Fig. 3) is given in this correction.

Fig. 3
figure 1

Purification and activation of prenisin produced by C. glutamicum. A Relative mCherry fluorescence normalized to OD (RFU/OD) of L. lactis NZ9000/pNZ-Pnis-mcherryLl grown o/N in the presence of supernatants (SN) of C. glutamicum CR099/pXMJ-nisZBTCCg. The producer was grown o/N in 2xTY with 2% Glc and 0.2 mM IPTG. B Purification of ammonium sulphate-precipitated SN proteins by cation exchange (CIEX) and subsequent reverse phase (RP) chromatography on the CIEX peak fraction. Indicated is absorbance at 214 nm (red) and conductance (mS/cm; black, in CIEX) or % of elution buffer (%B, black, in RP) over the elution volume. Boundaries of the peak fractions further analysed are marked with blue broken lines. CRFU/OD of L. lactis NZ9000/pNZ-Pnis-mcherryLl grown o/N in the presence of samples obtained at different steps during the purification of prenisin from SN shown in (A). prec: ammonium sulphate-precipitated SN proteins resuspended in pure H2O; CIEX and RP peak: peak fraction of the CIEX and RP chromatography. Prior to assays, samples were activated by incubation with trypsin (0.5 mg/ml for 3.5 h) and diluted 1:1000. As positive controls, the biosensor was grown in the presence of nisin Z at the indicated concentration. As negative controls, SN without trypsin treatment were included. D Mass spectrometry of the peak fraction obtained in RP chromatography in B with arbitrary peak intensity units (intensity [AU]) over mass/charge ratio (m/z). Values in A and C are mean ± SD of n = 3 independent cultures (A) or technical triplicates of one representative preparation (C)

Reference

  1. Weixler D, Berghoff M, Ovchinnikov KV, Reich S, Goldbeck O, Seibold GM, Wittmann C, Bar NS, Eikmanns BJ, Diep DB, Riedel CU. Recombinant production of the lantibiotic nisin using Corynebacterium glutamicum in a two-step process. Microb Cell Factories. 2022; 21(1):1-5.

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Correspondence to Christian U. Riedel.

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Weixler, D., Berghoff, M., Ovchinnikov, K.V. et al. Correction to: Recombinant production of the lantibiotic nisin using Corynebacterium glutamicum in a two‑step process. Microb Cell Fact 21, 24 (2022). https://doi.org/10.1186/s12934-022-01744-1

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  • DOI: https://doi.org/10.1186/s12934-022-01744-1