Antibody production by a protease-deficient strain of methylotrophic yeast, Ogataea minuta
© Kuroda et al; licensee BioMed Central Ltd. 2006
Published: 10 October 2006
At present the expression system of mammalian cells such as CHO has been adopted as the conventional method to produce antibody for pharmaceuticals. However a novel method of producing antibody has been sought after as a substitute for the costly production of antibody by mammalian cells. Therefore, we tried to construct a novel antibody production system by using methylotrophic yeast, O. minuta.
We constructed a protease-deficient strain and confirmed the secretion of full-length antibody by yeast. Improving the production of antibody should be a subject of further research.
We thank N.Kawashima, A. Oonishi, Y. Yamamoto, M. Tezuka and K. Sato for their valuable assistance with this research. We are also grateful to Dr. M. Tsukahara for helpful suggestions.
- Bourbonnais Y, Larouche C, Tremblay GM: Production of full-length human pre-elafin, an elastase specific inhibitor, from yeast requires the absence of a functional yapsin 1 (Yps1p) endoprotease. Protein Expr Purif. 2000, 20: 485-491. 10.1006/prep.2000.1338.View ArticleGoogle Scholar
- Wood CR, Boss MA, Kenten JH, Calvert JE, Roberts NA, Emtage JS: The synthesis and in vivo assembly of functional antibodies in yeast. Nature. 1985, 314: 446-449. 10.1038/314446a0.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd.