Kluyveromyces lactis SSO1 and SEB1 genes are functional in Saccharomyces cerevisiae and enhance production of secreted proteins when overexpressed
© Toikkanen et al; licensee BioMed Central Ltd. 2006
Published: 10 October 2006
The SEB1/SBH1 and the SSO genes encode components of the protein secretory machinery functioning at the opposite ends, ER translocation and exocytosis, respectively, of the secretory pathway of Saccharomyces cerevisiae. Overexpression of these genes can rescue temperature-sensitive (ts) growth defect of many sec mutants impaired in protein secretion. Their overexpression in wild-type yeast enhances production of secreted proteins in S. cerevisiae, which suggests that may be rate-limiting factors in this process.
The single copy genes KlSSO1 and KlSEB1 are both structurally and functionally conserved in evolution with their duplicated S. cerevisiae homologs.
- Toikkanen JH, Sundqvist L, Keränen S: Kluyveromyces lactis SSO1 and SEB1 genes are functional in Saccharomyces cerevisiae and enhance production of secreted proteins when overexpressed. Yeast. 2004, 21: 1045-1055. 10.1002/yea.1151.View ArticleGoogle Scholar
- Ruohonen L, Toikkanen J, Tieaho V, Outola M, Söderlund H, Keränen S: Enhancement of protein secretion in Saccharomycescerevisiae by overproduction of Sso protein, a late-acting component of the secretory machinery. Yeast. 1997, 13: 337-351. 10.1002/(SICI)1097-0061(19970330)13:4<337::AID-YEA98>3.0.CO;2-K.View ArticleGoogle Scholar
- Toikkanen JH, Miller KJ, Söderlund H, Jäntti J, Keränen S: The β subunit of the Sec61p endoplasmic reticulum translocon interacts with the exocyst complex in Saccharomyces cerevisiae. J Biol Chem. 2003, 278: 20946-20953. 10.1074/jbc.M213111200.View ArticleGoogle Scholar
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