Assessment of metabolic flux distribution in the thermophilic hydrogen producer Caloramator celer as affected by external pH and hydrogen partial pressure
© Ciranna et al.; licensee BioMed Central Ltd. 2014
Received: 10 February 2014
Accepted: 18 March 2014
Published: 28 March 2014
Caloramator celer is a strict anaerobic, alkalitolerant, thermophilic bacterium capable of converting glucose to hydrogen (H2), carbon dioxide, acetate, ethanol and formate by a mixed acid fermentation. Depending on the growth conditions C. celer can produce H2 at high yields. For a biotechnological exploitation of this bacterium for H2 production it is crucial to understand the factors that regulate carbon and electron fluxes and therefore the final distribution of metabolites to channel the metabolic flux towards the desired product.
Combining experimental results from batch fermentations with genome analysis, reconstruction of central carbon metabolism and metabolic flux analysis (MFA), this study shed light on glucose catabolism of the thermophilic alkalitolerant bacterium C. celer. Two innate factors pertaining to culture conditions have been identified to significantly affect the metabolic flux distribution: culture pH and partial pressures of H2 (P H2). Overall, at alkaline to neutral pH the rate of biomass synthesis was maximized, whereas at acidic pH the lower growth rate and the less efficient biomass formation are accompanied with more efficient energy recovery from the substrate indicating high cell maintenance possibly to sustain intracellular pH homeostasis. Higher H2 yields were associated with fermentation at acidic pH as a consequence of the lower synthesis of other reduced by-products such as formate and ethanol. In contrast, P H2 did not affect the growth of C. celer on glucose. At high P H2 the cellular redox state was balanced by rerouting the flow of carbon and electrons to ethanol and formate production allowing unaltered glycolytic flux and growth rate, but resulting in a decreased H2 synthesis.
C. celer possesses a flexible fermentative metabolism that allows redistribution of fluxes at key metabolic nodes to simultaneously control redox state and efficiently harvest energy from substrate even under unfavorable conditions (i.e. low pH and high P H2). With the H2 production in mind, acidic pH and low P H2 should be preferred for a high yield-oriented process, while a high productivity-oriented process can be achieved at alkaline pH and high P H2.
The need to circumvent environmental and social issues concerning the depletion of fossil fuels and the greenhouse gas emissions has led to exploration of alternative sources of energy. Hydrogen (H2) is considered as a promising energy carrier for the future because of its high energy content and non-polluting properties . Moreover, it can be employed as a non-fuel commodity in a variety of industrial chemo-physical processes for which the demand is increasing .
Dark fermentation is a potential carbon neutral process for production of H2 from organic substrates by mesophilic or thermophilic anaerobic microorganisms. In order to establish an economically viable biological process for H2 production the yield needs to be maximized . Mesophilic microorganisms are not capable of producing H2 at high yield being it reported in the range of 1-2 mol H2/mol hexose . On the other hand, thermophiles have shown great potential for H2 generation, mainly because of the more favorable thermodynamics of the reaction at elevated temperatures which allows to generate a limited variety of by-products and to reach the theoretical yield of 4 mol H2/mol hexose .
Caloramator celer, formerly known as Thermobrachium celere , is a strict anaerobic, alkalitolerant, thermophilic bacterium capable of converting C6 sugars to H2, CO2, acetate, ethanol and formate as major metabolites. Previous studies have shown that C. celer produces H2 at high yields both in a naturally occurring microbial community and in pure culture [7–9]. However, the distribution of the end metabolites can vary depending on the growth conditions allowing the accumulation of significant amounts of ethanol and formate with consequent reduction of H2 yield.
Two innate factors pertaining to culture conditions have been identified to have a prominent role in the distribution of metabolic fluxes during anaerobic fermentation in C. celer: partial pressures of H2 (P H2) and culture pH [8, 9]. The inhibitory effect on microbial growth and H2 production caused by H2 accumulation both in liquid and gaseous phases during the fermentation is a well known phenomenon and it has been demonstrated for several H2-producing organisms [9–13]. The fermentation medium can be easily supersaturated with H2 due to liquid-to-gas mass transfer limitations and this seems to be inevitable also at elevated temperatures [14, 15]. In these conditions H2 synthesis becomes thermodynamically unfavorable and consequently the disposal of accumulated reducing equivalents in the cell is mediated by a metabolic shift towards production of more reduced metabolites, such as lactate, ethanol, acetone, butanol, or alanine . As a consequence of the changes in the fermentation profile both H2 and ATP yields decrease. In previous studies C. celer achieved high H2 yields ( ≥ 3.3 mol H2/mol glucose) at low culture-to-headspace volume ratio and when H2 was periodically removed from the headspace [8, 9]. These observations suggest that in order to maximize the H2 yield in C. celer P H2 in the fermentation vessel should be maintained at low level.
Culture pH is another factor affecting the distribution of metabolic fluxes during glucose fermentation. However, there exists a certain disagreement on the optimal pH to employ for H2 production . Certainly, the optimal value needs to be studied case by case as it depends on the metabolic and physiological properties of the microorganism under investigation. Moreover, the ideal pH for cell growth may not be the same as the one for maximal H2 production .
Recently, a draft whole-genome sequence of C. celer has been obtained allowing to gain insight into the metabolic potential of this organism . In particular, genomic analysis revealed pathways involved in pyruvate catabolism and end-product synthesis. To get a better understanding of the fermentative metabolism of C. celer under controlled conditions, a combination of experimental data from batch cultures in continuous stirred-tank reactor (CSTR), recently available genomic data and methods of metabolic flux analysis (MFA) were employed to analyze flux distribution towards end-products, taking in special consideration the effect of two innate factors, pH and P H2, on hydrogen production.
Effect of culture pH on fermentative metabolism of C. celer
pH-controlled batch fermentations of C. celer on 5 g/l of glucose at different culture pH
μ max (h-1)
1.50 ± 0.05
1.34 ± 0.05
0.53 ± 0.01
0.45 ± 0.06
Biomass concentration (gCDW/l)
0.95 ± 0.01
0.95 ± 0.06
0.76 ± 0.01
0.42 ± 0.01
q glucose a (mmol/gCDW/h)
19.6 ± 1.4
20.6 ± 0.1
14.3 ± 0.1
14.0 ± 0.2
YX/ATP (gCDW/mol ATP)
15.4 ± 0.7
15.2 ± 0.4
6.8 ± 0.2
6.0 ± 0.4
YATP/S (mol ATP/mol glucose)
2.81 ± 0.02
3.05 ± 0.03
3.54 ± 0.33
3.60 ± 0.09
H2 accumulation (mmol H2/l)
68.0 ± 1.3
64.2 ± 1.1
106.0 ± 2.3
91.7 ± 0.4
Q H2 (mmol H2/l/h)
25.2 ± 1.2
21.0 ± 1.3
14.9 ± 0.2
13.0 ± 0.9
Product yield (mol/mol glucose)
1.29 ± 0.05
1.37 ± 0.07
1.04 ± 0.01
0.62 ± 0.02
1.90 ± 0.01
1.69 ± 0.03
2.97 ± 0.10
2.48 ± 0.03
1.04 ± 0.01
1.02 ± 0.04
1.33 ± 0.08
1.28 ± 0.04
0.45 ± 0.01
0.37 ± 0.11
0.14 ± 0.02
0.19 ± 0.01
0.35 ± 0.01
0.48 ± 0.05
0.20 ± 0.01
0.36 ± 0.03
2.60 ± 0.03
2.65 ± 0.13
3.36 ± 0.09
3.19 ± 0.02
2.96 ± 0.11
2.15 ± 0.30
6.83 ± 0.67
3.60 ± 0.14
Carbon recovery (%)
88.6 ± 1.1
99.8 ± 1.5
100.7 ± 2.8
93.5 ± 1.6
Redox recovery (%)
94.4 ± 0.6
99.2 ± 0.7
98.4 ± 3.2
93.6 ± 2.1
Besides growth, culture pH affected also the metabolite profile of C. celer during glucose fermentation. The highest H2 accumulation (106 mmol H2/l) and H2 yield (2.97 mol H2/mol glucose) were observed at pH 6, while at higher pH their values were about 33% lower (Table 1). In contrast, higher volumetric H2 productivity (Q H2) was obtained at alkaline conditions (25.2 mmol H2/l/h) and gradually decreased as the culture pH was reduced. The lower H2 production observed at pH 8 and 7 was accompanied by an increase of formate and ethanol yields, both being about 2-fold higher compared to those observed at pH 6 (Table 1). The lower RVEP (total molar reduction values of reduced end-products)  and the acetate-to-ethanol ratio calculated for fermentation at pH 8 and 7 reflected the increased production of formate and ethanol (Table 1).
Combined effect of culture pH and P H2 on fermentative metabolism of C. celer
Combined effect of pH and P H2 on growth and metabolite production profiles
pH-controlled batch fermentations of C. celer on 10 g/l of glucose at pH 7 and 6, with and without N 2 sparging
N2sparging ( Case I)
No sparging ( Case II)
N2sparging ( Case III)
No sparging ( Case IV)
P H2max (kPa)
μ max (h-1)
1.09 ± 0.09
1.09 ± 0.08
0.43 ± 0.03
0.38 ± 0.05
Biomass concentration (g/l)
0.99 ± 0.03
1.13 ± 0.01
1.02 ± 0.03
1.01 ± 0.01
YX/ATP (gCDW/mol ATP)
11.5 ± 0.3
9.3 ± 0.1
6.3 ± 0.4
7.9 ± 0.2
YATP/S (mol ATP/mol glucose)
3.24 ± 0.03
3.18 ± 0.02
3.4 ± 0.08
3.48 ± 0.17
Q H2 (mmol H2/l/h)
27.1 ± 1.5
33.0 ± 0.1
19.9 ± 0.8
20.6 ± 0.5
Product yield (mol/mol glucose)
0.71 ± 0.01
0.75 ± 0.03
0.72 ± 0.01
0.75 ± 0.01
2.32 ± 0.13
2.06 ± 0.13
3.10 ± 0.06
2.60 ± 0.03
1.24 ± 0.01
1.28 ± 0.01
1.47 ± 0.07
1.35 ± 0.02
0.37 ± 0.02
0.71 ± 0.12
0.14 ± 0.07
0.33 ± 0.06
0.27 ± 0.01
0.37 ± 0.01
0.10 ± 0.01
0.39 ± 0.03
2.87 ± 0.11
2.8 ± 0.12
3.31 ± 0.08
3.39 ± 0.03
4.5 ± 0.2
3.5 ± 0.1
14.2 ± 1.0
3.4 ± 0.3
Carbon recovery (%)
92.6 ± 0.6
94.0 ± 0.7
94.8 ± 4.2
100.3 ± 1.9
Redox recovery (%)
90.0 ± 0.6
97.5 ± 0.6
93.2 ± 4.1
102.3 ± 2.2
Both culture pH and P H2 influenced the carbon flux distribution and the end-product profile in C. celer during glucose fermentation. The beneficial effect of H2 removal from the reactor on H2 production was more evident at pH 6 (Case III and IV). In fact, at this pH, when N2 sparging was applied, H2 accumulation increased by about 26% and H2 yield by 19% (Case III) (Figure 1C; Table 2). On the other hand, at pH 7 (Case I and II) the effect of P H2 on H2 production was marginal with a 9% and 13% increase at low P H2 for H2 accumulation and H2 yield, respectively (Case I). In addition, applying N2 sparging did not affect Q H2 within the same pH level. At the same sparging condition acidic pH promoted higher H2 accumulation and H2 yield, while neutral pH favored higher Q H2 (Figure 1C; Table 2). In fact, when N2 sparging was applied (Case I and III) H2 accumulation increased by 32% and H2 yield by 33% as pH set-point was lowered from 7 to 6. Even so, the Q H2 at pH 7 was about 36% higher than at pH 6. A similar trend could be observed without N2 sparging (Case II and IV), although the differences between H2 accumulation and H2 yield at pH 6 and pH 7 were significant but less pronounced.
Carbon and electron flow at the pyruvate and acetyl-CoA nodes was affected by both pH and P H2. While acetate accumulation profile and yield were minimally influenced by the P H2, formate and ethanol synthesis significantly increased at both pH levels when H2 concentration was allowed to build up in the reactor (Case II and IV) (Figure 1D, 1E, 1F; Table 2). Specifically, formate accumulation and yield were about 2-fold higher without N2 sparging regardless of the culture pH (Case II and IV), whereas the effect of P H2 on ethanol production was more drastic at pH 6 (Case IV) with an increase of accumulation and yield of 2.5- and 4-fold, respectively. The ability of C. celer to produce reduced end-products (RVEP), i.e. H2 and ethanol, remained unchanged under different P H2 (Table 2). On the other hand, acetate-to-ethanol ratio showed that at the acetyl-CoA node the redirection of carbon and electron flow toward ethanol synthesis is less favorable at low P H2, especially at pH 6 (Case III) where the highest acetate-to-ethanol ratio of 14.2 was observed (Table 2).
Acidic pH (Case III and IV) promoted a slight increase of acetate accumulation and yield, whereas formate accumulation and yield were reduced by more than 2-fold at pH 6 both with and without gas stripping (Case III and IV) (Figure 1D, 1E; Table 2). A drastic reduction of ethanol synthesis occurred only at acidic pH with N2 sparging (Case III), while at high P H2 both ethanol accumulation and yield remained at high level regardless of the culture pH (Case II and IV) (Figure 1F; Table 2). The increase of RVEP from 2.8-2.9 at pH 7 (Case I and II) to 3.3-3.4 at pH 6 (Case III and IV) indicates that pH influenced the conversion of glucose to reduced end-products (H2 and ethanol). The flux at the acetyl-CoA node was affected by pH only when sparging was applied resulting in a 3-fold increase of the acetate-to-ethanol ratio at pH 6 (Case III), while no variation in the ratio was observed when H2 concentration was allowed to build up in the reactor.
Combined effect of pH and P H2 on ATP and redox levels
Intracellular ATP, NADH and NAD + concentrations, and NADH/NAD + ratio
N2sparging ( Case I)
No sparging ( Case II)
N2sparging ( Case III)
No sparging ( Case IV)
ATP (μmoles/g biomass)
0.097 ± 0.005
0.117 ± 0.003
0.046 ± 0.003
0.043 ± 0.003
0.254 ± 0.025
0.199 ± 0.017
0.029 ± 0.002
0.030 ± 0.002
NADH (μmoles/g biomass)
0.019 ± 0.004
0.021 ± 0.005
0.007 ± 0.002
0.011 ± 0.002
0.015 ± 0.003
0.025 ± 0.003
0.010 ± 0.002
0.015 ± 0.002
NAD+ (μmoles/g biomass)
0.239 ± 0.011
0.335 ± 0.032
0.231 ± 0.002
0.271 ± 0.001
0.201 ± 0.015
0.205 ± 0.038
0.005 ± 0.001
0.010 ± 0.001
0.08 ± 0.02
0.06 ± 0.02
0.03 ± 0.01
0.04 ± 0.01
0.07 ± 0.01
0.13 ± 0.01
1.77 ± 0.23
1.56 ± 0.39
In the exponential phase the NADH/NAD+ ratio measured with and without N2 sparging resulted to be similar at both pH levels (Table 3). However, at pH 7 the NADH/NAD+ ratio increased by 2-fold (from 0.06 to 0.13) during the stationary phase when P H2 reached about 56 kPa (Case II), while it remained unaffected when P H2 was kept low by N2 sparging (Case I). Surprisingly, a dramatic increase of the NADH/NAD+ ratio was observed during the stationary phase at pH 6 (Case III and IV). This was caused by an expected depletion of NAD+ pool rather than an increase in intracellular NADH concentration (Table 3).
Metabolic flux analysis under different pH and P H2
Acetyl-CoA is another critical intermediate in C. celer metabolism. Conversion of acetyl-CoA to acetate (v16) generates one extra ATP, while ethanol production is used as an electron sink for the oxidation of NADH (v17). Although production of acetate was generally favored, ethanol flux varied depending of the experimental conditions (Figure 3C). Applying N2 sparging to prevent H2 building up in the reactor promoted a lower ethanol flux in the exponential phase (Case I and III). In particular, the flux directed to ethanol was 3- and 2-fold lower for Case I and III respectively, and similarly the v16/v17 ratio was 3- and 2.5-fold higher suggesting that low P H2 favored acetate over ethanol production in the exponential phase. Ethanol flux in the exponential phase was lower at pH 7 compared to pH 6 at both sparging conditions. However, while at pH 6 (Case III and IV) the ethanol production completely ceased in the stationary phase, at pH 7 (Case I and II) it started increasing at the end of the exponential phase resulting in about 25% of the flux to be directed to ethanol regardless of the P H2 in the reactor. Since conversion of acetyl-CoA to ethanol requires NADH as reduced cofactor, it competes with NADH-dependent H2 production for the use of reducing equivalents. Figure 5B supports this scenario showing that in C. celer NADH-dependent H2 flux (v18) estimated at different growth phases was negatively correlated with ethanol flux (v17).
Data from MFA was also used to analyze the H2 flux and how its two components, the NADH-dependent (v18) and the Fd-dependent (v19) H2 production, contribute to it (Figure 3D). In the exponential phase N2 sparging had a great effect on the NADH-dependent H2 flux (v19) that increased from 23.4 (Case II) to 66.5% (Case I) of the total H2 flux at pH 7 and from 20.9 (Case IV) to 40.2% (Case III) at pH 6. However, no evident trend can be identified in the distribution of H2 flux with respect of the culture pH in this growth phase. In general, with the exception of Case I, during the exponential phase Fd-dependent proton reduction (v19) was the favorite reaction for H2 synthesis. As the fermentation entered in the stationary phase, at pH 7 (Case I and II) Fd-dependent H2 flux (v19) was almost twice the NADH-dependent H2 flux (v18), while at pH 6 (Case III and IV) the H2 flux was equally distributed between the Fd-dependent (v19) and the NADH-dependent reaction (v18). When comparing the overall H2 flux (v18 + v19) normalized by glucose uptake (v1) it is clear that low P H2 achieved by N2 sparging (Case I and III) promoted higher H2 production in C. celer, whereas when H2 was allowed to accumulate in the reactor (Case II and IV) H2 evolution suffered (Figure 3D). Similarly, running the fermentation at acidic pH (Case III and IV) instead of at neutral pH (Case I and II) increased the disposal of reducing equivalent through H2 production, especially in the stationary phase when fluxes of the competing reactions (v14 and v17) were minimal (Figure 5C).
Generation and consumption of reducing equivalents in C. celer
During glucose catabolism reducing equivalents are generated in the form of NADH or Fdred. These cofactors need to be reoxidized allowing the glycolytic flux to proceed. During fermentation this can be achieved through different reactions involving the synthesis of reduced molecules such as H2, ethanol, lactate, butyrate and alanine. Specifically, C. celer can produce NADH during the oxidation of glyceraldehyde-3-phosphate by an NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH, TCEL_00702) and Fdred in the oxidation of pyruvate to acetyl-CoA by pyruvate:ferredoxin oxidoreductases (PFOR, TCEL_02202-02206/TCEL_01566) (Figure 2A; Additional file 1: Table S1). While in C. celer glycolysis (Embden-Meyerhof pathway) yields 2 NADH per one glucose metabolized, generation of Fdred is dependent on flux distribution at the pyruvate node. At this node pyruvate is non-oxidatively dissimilated to acetyl-CoA and formate by pyruvate formate lyase (PFL, TCEL_00503) or oxidatively decarboxylated to acetyl-CoA by PFOR. Only the latter generates Fdred and when 100% of the flux goes through PFOR, generation of reducing equivalents is maximized resulting in the formation of 2 NADH and 4 Fdred. If all reduced electron carriers were recycled by hydrogenases, the complete oxidation of one molecule of glucose would yield 4 H2 molecules. The genome of C. celer contains two clusters coding for putative [FeFe]-hydrogenases (TCEL_00581-00584/TCEL_01273-01277) and one cluster coding for a putative [NiFe]-hydrogenase (TCEL_00187-00205) (Figure 2A; Additional file 1: Table S1). Based on similarity of the gene organization within the clusters and the high homology with the cytosolic enzyme complex of Caldanaerobacter subterraneus subsp. tengcongensis , the two [FeFe]-hydrogenases most likely utilize NADH as an electron donor for proton reduction. The [NiFe]-hydrogenase is predicted to be a multimeric Fd-dependent membrane-bound enzyme similar to the MBH complex characterized in Pyrococcus furiosus . In this species MBH couples the oxidation of Fdred with energy conservation by forming an ion gradient that can be used to generate ATP via a membrane-bound ATP synthase.
However, recycling reducing equivalent through H2 production is not always thermodynamically favorable. At a given temperature the oxidation of reduced cofactors (particularly of NADH) by hydrogenases is a function of the H2 concentration . Supersaturation of the aqueous phase with H2 can easily occur due to limitation of liquid-to-gas mass transfer rate [14, 15]. As a consequence, at high H2 concentrations the disposal of accumulated reducing equivalents in the cell is mediated by a metabolic shift towards production of more reduced metabolites such as ethanol, lactate, and alanine. The genome analysis revealed that in C. celer the only alternative for NADH oxidation is ethanol production, since no lactate dehydrogenase nor NAD:ferredoxin oxidoreductase (NFOR) were identified (Figure 2A; Additional file 1: Table S1). In addition, although C. celer encodes a complete pathway for butyrate synthesis that would consume NADH, no butyrate was detected as end-product in this study. Ethanol production mediated by putative bifunctional acetaldehyde/alcohol dehydrogenase (TCEL_01373) and alcohol dehydrogenase (TCEL_00064) has been shown to increase at high P H2 validating the hypothesis that this pathway serves as an alternative to hydrogenase for NADH reoxidation [8, 9].
As in C. celer the only possibility for Fdred oxidation relies on the activity of the ferredoxin-dependent MBH complex, the distribution of carbon and electron flow at the pyruvate nodes dictates the size of the Fdred pool available for H2 production. Despite the similar standard Gibbs energy (ΔGo´) , the reaction catalyzed by PFOR is expected to be less favorable at high H2 concentration due to the increased Gibbs energy (ΔG′) of the ferredoxin oxidation by H2 production, whereas the thermodynamics of the reaction catalyzed by PFL does not change. Therefore, in C. celer the branched pyruvate node can serve as a safety valve relieving the cell from the burden of ferredoxin reoxidation in unfavorable conditions and thus avoiding a decrease in the metabolic flux.
Effect of culture pH on growth and fermentative metabolism of C. celer
In previous studies performed under non-controlled conditions the optimal initial pH for H2 accumulation in C. celer was observed to be 8.2 at 67°C (approximately 9.0 at room temperature) [8, 9] in accordance with the optimal pH for growth . However, in non-pH-controlled conditions the dynamic pH profile caused by the production of organic acids masked the real effect of pH on the metabolism of C. celer. In the current study, pH-controlled fermentations using both 5 and 10 g/l of glucose showed that alkaline to neutral pH (8 and 7 at room temperature) clearly favor high growth rates in C. celer confirming the alkalitolerant nature of this organism. On the other hand, at moderately acidic pH (6 and 5.5 at room temperature) the μ max was reduced by 2 to 3-fold (Table 1, 2) regardless of the P H2 in the system. However, with 10 g/l of glucose in the medium, growth ceased before complete glucose depletion and the biomass yield was identical for all the conditions (Table 2). This could be caused by nutrient limitation or by inhibiting conditions such as increased osmolality and accumulation of by-products in the culture [13, 22]. When C. celer grew at high rates in alkaline/neutral conditions, biomass formation was more energy-efficient despite the lower overall ATP recovery from glucose (Table 1, 3). In contrast, at acidic pH the growth rates were reduced and inefficient biomass formation was observed despite the more effective energy recovery. Given the alkalitolerant nature of C. celer, it is likely that at suboptimal extracellular pH this organism needs to maintain the intracellular pH within the physiological range. This goal can be achieved by H+-ATPase which exports protons at the expense of ATP hydrolysis. The activity of this enzyme is known to increase at low intracellular pH, thus consuming a substantial portion of the intracellular ATP produced via substrate level phosphorylation [23, 24]. Based on the intracellular ATP levels measured at different culture pH conditions (Table 3) and the flux of ATP hydrolysis (v21) (Figure 2B), it can be hypothesized that in C. celer at lower pH non-growth-associated ATP consumption increases most probably to achieve cytoplasmic pH homeostasis.
An analysis of the end-product distribution clearly indicated that C. celer exhibited different metabolic patterns depending on the culture pH. Surprisingly, at moderately acidic pH conversion of glucose to H2 was more favorable ( > 2 mol H2/mol glucose) than at alkaline to neutral pHs ( < 2 mol H2/mol glucose) as a consequence of the lower synthesis of other reduced by-products, i.e. formate and ethanol (Tables 1, 2). On the other hand, volumetric H2 productivity was directly correlated with μ max, both increasing as pH was shifted to alkaline pH. Notably, a transition in the metabolic behavior of C. celer was observed between pH 7 and 6 (Tables 1, 2). In particular, both the formate yield and the formate flux during exponential phase were more than 2-fold higher at pH 7 regardless of the P H2 in the reactor (Table 2; Figures 2B, 3B). A widespread tendency among PFL-encoding organisms to accumulate formate during glucose fermentation as culture pH becomes more neutral/alkaline was observed, albeit with different magnitude, both in pure [25–32] and mixed cultures [33, 34]. In absence of a formate hydrogen lyase (FHL) in C. celer and other strictly anaerobic bacteria the synthesis of formate by PFL competes with generation of H2 .
Although at this stage the exact mechanism of this metabolic shift triggered by culture pH in C. celer is unknown, several hypotheses can be proposed: i) a simultaneous anabolic and catabolic role of PFL, as reported in closely related clostridia , would justify the higher flux through PFL observed at elevated growth rates in this (Figure 4A) and previous studies  possibly to meet energy and anabolic demands at optimal pH for growth; ii) since pyruvate can trigger its own utilization by PFL participating as allosteric effector in the activation of this enzyme [36, 37] and acetyl-CoA can inhibit PFOR [38–40], at elevated growth rates observed at neutral/alkaline pH higher pyruvate and acetyl-CoA fluxes favored the reaction catalyzed by PFL, whereas pyruvate was mainly directed to PFOR under reduced pyruvate and acetyl-CoA fluxes observed at lower growth rates in acidic conditions and in the transition from the exponential to the stationary phase (Figures 2B, 3B, 4B); iii) the activity of PFL is optimal at pH slightly above 7, but reduced under acidic conditions [37, 41]; iv) while the ΔGo´ of the reaction catalyzed by PFL is not affected by pH, the reoxidation of Fdred via proton reduction necessary to drive the oxidative pyruvate dissimilation by PFOR, becomes less favorable as the pH increases . Overall, given the direct correlation between flux distribution at the pyruvate node during exponential phase and H2 yield (Figure 4B), carbon and electron flow should be channeled through PFOR instead of PFL to maximize H2 synthesis.
Unlike formate, ethanol production does not seem to be dependent on pH culture. In fact, at 5 g/l of glucose with N2 sparging the highest ethanol yield (0.48 mol/mol glucose) was achieved at pH 7 and the lowest (0.20 mol/mol glucose) at pH 6 (Table 1). However, ethanol yields at pH 8 and 5.5 were similar. Moreover, at high P H2 no difference was observed between pH 7 and 6 (Table 2). This suggests that regulation of carbon flux through the acetyl-CoA branch point could potentially serve as a means of controlling the disposal of reducing equivalents necessary to maintain an internal redox balance.
Effect of P H2 on growth and fermentative metabolism of C. celer
The results obtained in this study show that under controlled conditions P H2 does not affect the growth of C. celer. Both biomass formation and bioenergetics parameters (YX/ATP and YATP/S) were the same regardless the P H2 (Figure 1A; Table 2). Also growth rate and consequently volumetric H2 productivity were not dependent on P H2 (Table 2). Under given conditions, the growth rate is dependent on the glycolytic flux which, in turn, is a function of the activity of GAPDH. The activity of GAPDH is negatively affected by an increase of the NADH/NAD+ ratio [42, 43]. During batch cultivations the NADH/NAD+ in C. celer was low at exponential phase at both pH (Table 3), suggesting a complete NADH reoxidation regardless of the P H2. As a consequence, the flux through GAPDH (v4) was not inhibited by high P H2 (Figure 2B). Unlike other anaerobic bacteria which employ alternative pathways to H2 production for the reoxidation of NADH (e.g. ethanol and lactate production) only in response to high NADH/NAD+ [43, 44], in C. celer the ethanol production started already in the exponential phase (Figure 1F) preventing the NADH/NAD+ to increase and inhibit GAPDH. As a result, in the conditions at which NADH-dependent H2 formation is supposed to be inhibited by high P H2 (Case II and IV) ethanol yield is higher (Table 2). NADH was maintained at low levels also in the stationary phase (Table 3). At this stage reoxidation of NADH through ethanol synthesis followed a pH-dependent pattern as at pH 7 ethanol flux (v17) and accumulation were still significant, while at pH 6 almost ceased (Figures 1F, 3C).
The pyruvate node is another important metabolic point for the management of reducing equivalents. Indeed, at high P H2 the overall formate yield and its flux (v14) in the exponential phase were higher suggesting that the non-oxidative reaction catalyzed by PFL serves as an alternative route for pyruvate dissimilation at high H2 concentration. In fact, while acetyl-CoA production by PFOR produces one pair of reducing equivalents (Fdred), in the PFL reaction the reducing equivalents remain with the product formate. Thus, regulation of carbon flux at the pyruvate node could potentially serve as an effective means of controlling the disposal of reducing equivalents necessary to maintain an internal redox balance. Additionally, this electron rerouting was pH-dependent since in Case II (pH 7 without N2 sparging) formate was accumulated also in the stationary phase and 25.9% of the flux at the pyruvate node was directed through PFL, whereas in all the other conditions formate accumulation and flux almost ceased at this stage of growth. Increased formate yield in response to high P H2 was reported also for Clostridium thermocellum [45, 46]. Formate production has never been previously proposed as a pathway to control redox balance in the cell, probably due to the scarce occurrence of a similarly branched pyruvate node in other strict anaerobes.
Eqs. (1-3) direct 100% of the carbon flux through PFOR (v15), whereas Eqs. (3-4) direct the flux entirely through PFL (v14). Conversion of glucose to acetate by Eq. (1) allows for maximal ATP generation (4 ATP) by completely relying on hydrogenase activity to regenerate reduced cofactors (NADH and Fdred). However, at high H2 concentration hydrogenases are inhibited and less-energy efficient pathways for disposal of reducing equivalents need to be activated to sustain the catabolic flux. Indeed, at 70°C and 1 M of dissolved H2 this reaction is the least thermodynamically favorable (ΔG′70°C = -153.1 kJ), but becomes more favorable as H2 concentration decreases (Additional file 2: Figure S1). The metabolic setup of C. celer allows for a reaction yielding 3 ATP (Eq. (4)) whose ΔG′70°C is completely independent of the H2 concentration (Additional file 2: Figure S1). Conversely, reactions thermodynamically independent of the H2 concentration used by other fermentative bacteria for balancing high NADH/NAD+ (e.g. ethanol and lactate synthesis) yield only 2 ATP [12, 43, 44]. Thus Eq. (4) ensures C. celer a higher energy-recovery from glucose breakdown and unaltered glycolytic flux even under unfavorable conditions for H2 production. In addition, C. celer can potentially still obtain 4 ATP through Eq. (3) which is slightly more favorable at high H2 concentrations than Eq. (1) (Additional file 2: Figure S1).
As shown by the inverse correlation between ethanol and formate fluxes with the two components of H2 flux (Figure 5A, B, C), ethanol and formate production serves as an alternative to H2 production for maintaining the redox balance in the cell when hydrogenases are inhibited: ADH assists NADH-dependent hydrogenase to keep low NADH levels, while PFL simultaneously supplies acetyl-CoA and stores reducing equivalents in formate thus regulating the pool of Fdred available for Fd-dependent hydrogenase. Given the stoichiometry of the fermentations and the distribution of the fluxes, C. celer utilizes a combination of the putative pathways to balance anabolic and catabolic requirements as well as intracellular redox state depending on the conditions. One exception can, however, be observed in Case III (pH 6 with N2 sparging) when C. celer entered the stationary phase. Under these conditions an equal contribution by the two components of the H2 flux and a normalized H2 flux close to the theoretical stoichiometric coefficient of 4 were observed (Figure 3D). Since no ethanol and formate production as well as biomass synthesis were detected, the fermentation proceeded according to Eq. (1). As previously observed , in C. celer the complete reoxidation of both Fdred and NADH through H2 production occurred at low pH and P H2 when glucose was used for non-growth associated cell maintenance.
Combining experimental results from batch fermentations, with genome analysis, reconstruction of central carbon metabolism and metabolic flux analysis (MFA), this study shed light on glucose catabolism of the thermophilic alkalitolerant bacterium C. celer. This organism possesses a flexible fermentative metabolism that allows efficient energy harvesting from substrate even under unfavorable conditions (i.e. low pH and high P H2). Two innate factors pertaining to culture conditions have been identified to significantly affect the metabolic flux distribution: culture pH and P H2.
Overall, at alkaline to neutral pH the rate of biomass synthesis of C. celer is maximized and the flux at the pyruvate node mainly directed to PFL suggesting a higher activity of this enzyme as well as a possible role in anabolic metabolism. On the other hand, at acidic pH the lower growth rate and the less efficient biomass formation are accompanied with a more efficient energy recovery from the substrate indicating a high cell maintenance requirement possibly to sustain intracellular pH homeostasis. Higher H2 yields were associated with fermentation at acidic pH as a consequence of lower synthesis of other reduced by-products such as formate and ethanol. In contrast, P H2 does not affect growth of C. celer on glucose. At high P H2 the cellular redox state was balanced by rerouting the flow of carbon and electrons to ethanol and formate production allowing unaltered glycolytic flux and growth rate, but resulting in a decreased H2 synthesis. Overall, with the optimization of H2 production in mind, C. celer offers the flexibility of shifting from a high yield-oriented process (at acidic pH with N2 sparging) to a high productivity-oriented process (at alkaline pH without N2 sparging). In particular, the tolerance exhibited by C. celer towards H2 build-up in the reactor is attractive because H2 production should be achieved preferably without the need for sparging gas to reduce capital costs for the gas-upgrading process . The metabolic control and regulation of the pyruvate node as well as the genetic, metabolic and physiological traits that allow C. celer to withstand high P H2 merit further studies.
Materials and methods
Microorganism and medium
Caloramator celer strain JW/YL-NZ35, former Thermobrachium celere (equivalent to DSMZ 8682 and ATCC 700318), was obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (Braunschweig, Germany). C. celer was cultivated in a modified ATCC 2072 medium.
(http://www.lgcstandards-atcc.org/~/media/4866DB6C29C44938B4B62542D2152259.ashx) containing (per liter): KH2PO4 0.75 g; Na2HPO4 · 2H2O 1.53 g; KCl 1 g; (NH4)2SO4 0.5 g; NH4Cl 0.5 g; MgCl2 · 6H2O 0.1 g; CaCl2 · 6H2O 0.11 g; FeSO4 · 7H2O 0.2 g; cystein-HCl 0.2 g; resazurin 0.001 g; trace element solution 10 ml; vitamin solution 10 ml; yeast extract 2 g; tryptone 2 g; glucose 5 or 10 g. Stock solutions under nitrogen atmosphere containing glucose, cystein-HCl, MgCl2 · 6H2O, CaCl2 · 6H2O, FeSO4 · 7H20 and vitamins were sterilized separately and added anaerobically to autoclaved medium at the required concentrations. Routine subcultures and inoculum development were conducted in 250 ml serum bottles containing 50 ml of medium at 67°C.
Cultures were grown in a jacketed, 3-liter bioreactor equipped with an ADI 1025 Bio-Console and an ADI 1010 Bio-Controller (Applikon, Schiedam, The Netherlands) at a working volume of 1 l. The initial pH was maintained at 67°C by automatic titration with 4 M NaOH. The temperature was thermostatically kept at 67 ± 1°C and the stirring rate was set to 250 rpm. A condenser with 5°C cooling water was fitted to the bioreactor's headplate. Prior to inoculation, the medium was sparged with N2 and supplemented with an anoxic solution of cysteine-HCl at a final concentration of 0.2 g/l to make the medium completely anaerobic along with the other stock solutions. Glucose was used as a primary substrate in all experiments at an initial concentration of 5 or 10 g/l. The medium was inoculated with 50 ml (5% v/v) of a culture in the exponential phase.
The effect of culture pH on the fermentative metabolism of C. celer was studied at four different pH values (8, 7, 6 and 5.5) measured at room temperature. In this experiment the bioreactor was constantly sparged with N2 at 100 ml/min. Glucose was added as substrate in concentration of 5 g/l. The effect of P H2 at two pH levels (7 and 6) was investigated by applying two experimental conditions: continuous N2 sparging at 100 ml/min for continuous removal of produced H2 (Case I at pH 7 and Case III at pH 6) and in absence of N2 sparging with the bioreactor's gas outlet open to allow accumulation of H2 in the headspace at atmospheric pressure (Case II at pH 7 and Case IV at pH 6). In the latter experimental condition the overall gas production was obtained by measuring the volume of displaced NaHCO3-saturated solution in a graduated cylinder. In this experiment glucose was added as substrate in concentration of 10 g/l. Gas samples from the headspace for H2 and CO2 determination and culture samples for monitoring growth, substrate consumption and metabolite formation were regularly withdrawn during growth. Samples for the measurement of intracellular ATP, NADH and NAD+ concentrations were collected as described earlier  during the exponential and stationary phase of the cultivations supplemented with 10 g/l glucose. All the experimental conditions were performed in biological duplicates and included negative controls omitting glucose.
Headspace samples were analyzed for H2 and CO2 concentration by gas chromatography, using a dual channel CP-4900 Micro-GC (Varian, Middelburg, The Netherlands) as described earlier . The results were analyzed with a Galaxie Chromatography Workstation (v.18.104.22.168). Cell concentrations were determined by measuring the absorbance at 620 nm using a spectrophotometer (Ultrospec 2100 pro, Amesham Biosciences, UK). Cell dry weight (CDW) was determined as described earlier . Glucose, acetate, formate, ethanol and butyrate were analyzed by HPLC (Waters, Milford, USA) on an Aminex HPX-87H ion exchange column (Bio-Rad, Hercules, USA) at 45°C, with 5 mM H2SO4 (0.6 ml min-1) as the mobile phase. The column was equipped with a refractive index detector (RID-6A, Shimadzu, Japan).
Cell culture samples (1 ml) were collected in the exponential and stationary phase of growth for NADH and NAD+ determination. Samples were immediately quenched by transferring them to a microcentrifuge tube containing 1 ml of ice and centrifuged for 1 min at 12,100 g. The pellets were immediately frozen and stored at -80°C until further analysis. Intracellular concentrations of NADH and NAD+ were determined by a cyclic assay as described earlier . Intracellular levels of NADH and NAD+ were expressed per 1 g of CDW.
Measurement of ATP
Cell culture samples (1 ml) were collected in the exponential and stationary phase of growth for ATP determinations. Samples were collected in screw-cap microcentrifuge tubes containing 1 ml of ice-cold chloroform and immediately frozen into liquid nitrogen. Samples were stored at -80°C until further analysis. Intracellular concentrations of ATP were measured with an ATP Bioluminescence assay kit HSII (Roche Molecular Biochemicals, Germany) as described earlier . Intracellular levels of ATP were expressed per 1 g of CDW.
H2 accumulation (mmol H2/l) was calculated in two different ways depending on the experimental design. When N2 sparging was applied the calculations were based on the flow rate of the influent N2 gas and the percentages of H2 and CO2 in the effluent gas, whereas when N2 sparging was not applied the flow rate of the effluent gas was measured by the liquid displacement method using a NaCO3-saturated solution to avoid any further CO2 solubilization. Molar H2 and CO2 were calculated using the ideal gas law based on their concentration in the effluent gas. Volumetric H2 productivity (Q H2; mmol H2/l/h) was determined from the slope of the straight line obtained by plotting the cumulative H2 (mmol H2/l) against the time (h) during exponential growth.
Carbon balances were calculated from the total amount of carbon-containing products formed (in C-mol) and the amount of sugar consumed (in C-mol). Electron balances were calculated after multiplying the amount of each metabolite and sugar by the corresponding degree of reduction (in mol electrons per C-mol). The chemical formula of biomass was assumed to be CH1.8O0.5 N0.2. Metabolite yields as well as carbon and electron balances were calculated by subtracting the background metabolite production in control cultivations (i.e. cultivation without the substrate) and the precultures’ carryover metabolites from the results.
where r is the specific production rate (mmol/gCDW/h), C is the substrate or metabolite concentration (mM) and X is the biomass (gCDW).
In silico model construction and metabolic flux analysis
The genome of C. celer strain JW/YL-NZ35 has been recently sequenced and a high quality draft sequence has been annotated as described earlier . The available genomic data was used to reconstruct the central carbon metabolism of C. celer and to build a stoichiometric model including glycolytic pathway, pentose phosphate pathway, end product synthesis pathways, transport mechanisms and biomass synthesis. In order to define each reaction and metabolite in the network, genes encoding for putative enzymes involved in the aforementioned pathways were identified by manual curation of the data from the annotated genome by a combination of sequence alignments , gene context analysis, database and literature searches. In addition, physiological evidences were used either when association between genes and reactions could not be established or when a reaction was removed from the network despite a corresponding gene being identified in the genome. The final model consisted of 24 reactions and 20 intracellular metabolites (Additional file 1: Table S1).
where Vc is the calculated flux vector, Gc * is pseudo-inverse matrix of the calculated reactions, Gm is the matrix of the measured reactions and Vm the measured flux vector. The matrix of MFA model was solved with MATLAB R2013a (The MathWorks, Inc., Natick, USA).
Alessandra Radici is kindly acknowledged for language editing of the manuscript. AC acknowledges support from the Academy of Finland (grants no.126974, 139830, 272602), the Finnish Doctoral Programme in Environmental Science and Technology (EnSTe) and Tampere University of Technology Graduate School. SSP acknowledges support from the Swedish Research Council (VR).
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