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Table 3 Intracellular ATP, NADH and NAD + concentrations, and NADH/NAD + ratio

From: Assessment of metabolic flux distribution in the thermophilic hydrogen producer Caloramator celer as affected by external pH and hydrogen partial pressure

  pH 7 pH 6
N2sparging ( Case I) No sparging ( Case II) N2sparging ( Case III) No sparging ( Case IV)
ATP (μmoles/g biomass)     
  Exponential phase 0.097 ± 0.005 0.117 ± 0.003 0.046 ± 0.003 0.043 ± 0.003
  Stationary phase 0.254 ± 0.025 0.199 ± 0.017 0.029 ± 0.002 0.030 ± 0.002
NADH (μmoles/g biomass)     
  Exponential phase 0.019 ± 0.004 0.021 ± 0.005 0.007 ± 0.002 0.011 ± 0.002
  Stationary phase 0.015 ± 0.003 0.025 ± 0.003 0.010 ± 0.002 0.015 ± 0.002
NAD+ (μmoles/g biomass)     
  Exponential phase 0.239 ± 0.011 0.335 ± 0.032 0.231 ± 0.002 0.271 ± 0.001
  Stationary phase 0.201 ± 0.015 0.205 ± 0.038 0.005 ± 0.001 0.010 ± 0.001
NADH/NAD+ (mol/mol)     
  Exponential phase 0.08 ± 0.02 0.06 ± 0.02 0.03 ± 0.01 0.04 ± 0.01
  Stationary phase 0.07 ± 0.01 0.13 ± 0.01 1.77 ± 0.23 1.56 ± 0.39