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Table 3 Intracellular ATP, NADH and NAD + concentrations, and NADH/NAD + ratio

From: Assessment of metabolic flux distribution in the thermophilic hydrogen producer Caloramator celer as affected by external pH and hydrogen partial pressure

 

pH 7

pH 6

N2sparging ( Case I)

No sparging ( Case II)

N2sparging ( Case III)

No sparging ( Case IV)

ATP (μmoles/g biomass)

    

  Exponential phase

0.097 ± 0.005

0.117 ± 0.003

0.046 ± 0.003

0.043 ± 0.003

  Stationary phase

0.254 ± 0.025

0.199 ± 0.017

0.029 ± 0.002

0.030 ± 0.002

NADH (μmoles/g biomass)

    

  Exponential phase

0.019 ± 0.004

0.021 ± 0.005

0.007 ± 0.002

0.011 ± 0.002

  Stationary phase

0.015 ± 0.003

0.025 ± 0.003

0.010 ± 0.002

0.015 ± 0.002

NAD+ (μmoles/g biomass)

    

  Exponential phase

0.239 ± 0.011

0.335 ± 0.032

0.231 ± 0.002

0.271 ± 0.001

  Stationary phase

0.201 ± 0.015

0.205 ± 0.038

0.005 ± 0.001

0.010 ± 0.001

NADH/NAD+ (mol/mol)

    

  Exponential phase

0.08 ± 0.02

0.06 ± 0.02

0.03 ± 0.01

0.04 ± 0.01

  Stationary phase

0.07 ± 0.01

0.13 ± 0.01

1.77 ± 0.23

1.56 ± 0.39