Quantitative metabolomics analysis of amino acid metabolism in recombinant Pichia pastoris under different oxygen availability conditions
© Carnicer et al.; licensee BioMed Central Ltd. 2012
Received: 23 February 2012
Accepted: 22 May 2012
Published: 15 June 2012
Environmental and intrinsic stress factors can result in the global alteration of yeast physiology, as evidenced by several transcriptional studies. Hypoxia has been shown to have a beneficial effect on the expression of recombinant proteins in Pichia pastoris growing on glucose. Furthermore, transcriptional profiling analyses revealed that oxygen availability was strongly affecting ergosterol biosynthesis, central carbon metabolism and stress responses, in particular the unfolded protein response. To contribute to the better understanding of the effect and interplay of oxygen availability and foreign protein secretion on central metabolism, a first quantitative metabolomic analysis of free amino acids pools in a recombinant P. pastoris strain growing under different oxygen availability conditions has been performed.
The values obtained indicate significant variations in the intracellular amino acid pools due to different oxygen availability conditions, showing an overall increase of their size under oxygen limitation. Notably, even while foreign protein productivities were relatively low (about 40–80 μg Fab/gDCW·h), recombinant protein production was found to have a limited but significant impact on the intracellular amino acid pools, which were generally decreased in the producing strain compared with the reference strain. However, observed changes in individual amino acids pools were not correlated with their corresponding relative abundance in the recombinant protein sequence, but to the overall cell protein amino acid compositional variations.
Overall, the results obtained, combined with previous transcriptomic and proteomic analyses provide a systematic metabolic fingerprint of the oxygen availability impact on recombinant protein production in P. pastoris.
Pichia pastoris has emerged as a workhorse for the production of recombinant proteins[1–4]. Moreover, the development of both synthetic and systems biotechnology tools specific for this cell factory platform[5–13], has opened new opportunities for metabolic engineering, as well as rational design and optimization of media composition and culture conditions.
Recombinant protein overproduction often results in a metabolic burden. Such effect may be reflected on process parameters such maximum growth rate, biomass yield or specific substrate consumption of yeast cells[4, 14–17], thus suggesting a potential impact on the cell’s energy metabolism, possibly derived from higher maintenance requirements. Furthermore, production of recombinant proteins may cause cellular stress due to unfolded proteins and unsuitable or inefficient secretion, which, in turn, may negatively affect cell growth, even at relatively low expression levels[15, 20], that is, at the product yields range where effects derived from increased energy and precursor demands for protein synthesis on cell growth should be theoretically negligible. In this context, limited but significant alterations in the carbon flux distribution over the central metabolism have been recently reported[21–24]. Also, amino acid supplementation of the growth medium has been shown to partially unburden cellular metabolism during recombinant protein production in yeast[23, 25–27]. Interestingly, such studies in P. pastoris have provided novel evidence that the adaptation of the central metabolism to recombinant protein production can not only be explained by an increased drain of precursors for protein synthesis. Indeed, amino acids are not only important precursors for protein synthesis but also participate in the regulation of major metabolic pathways. Glutamic and Aspartic acid for instance, are components of the aspartate/malate redox shuttle and their concentrations may indirectly impact on the rate of oxidation of glycolytic NADH.
We have previously reported the beneficial impact of hypoxia conditions on recombinant protein production in P. pastoris. The physiological bases of this beneficial effect were further investigated in a recent multilevel study including transciptome, proteome and metabolic flux analyses. These studies allowed to gather information on the biological processes involved in the adaptation to hypoxia and their relation with extracellular recombinant protein production in P. pastoris. However, the potential effect of oxygen availability and/or recombinant protein production on the intracellular metabolite levels (particularly of the amino acid precursors used for protein synthesis) remains to be elucidated.
To investigate the potential impact of foreign protein expression and secretion on amino acid metabolism, the free intracellular amino acids pools were analyzed in carbon limited chemostat cultivations at a fixed growth rate and different oxygenation conditions, using a recombinant P. pastoris strain secreting an antibody Fab fragment (E). The results are compared to the reference (non-producing) strain (C) and further combined with the metabolic fluxes related to protein synthesis and global transcriptome dataset from our previous study performed with the same strains and analogous cultivation conditions. Overall, this study aimed at understanding further the behaviour of the variations in intracellular amino acid levels as a result of the different oxygenation conditions employed, as well as gaining further insight in the potential interactions between energy metabolism and amino acid metabolism and, how such interactions may be perturbed by heterologous protein secretion in P. pastoris.
Results and discussions
Growth and recombinant protein secretion in recombinant P. Pastoris
Summary of macromolecular culture parameters
40 ± 5
82 ± 2
74 ± 9
−1.00 ± 0.02
−1.28 ± 0.03
−1.72 ± 0.05
−1.01 ± 0.02
−1.37 ± 0.03
−1.56 ± 0.04
−2.35 ± 0.06
−2.01 ± 0.07
−2.01 ± 0.15
−2.44 ± 0.07
−1.99 ± 0.08
−1.81 ± 0.13
2.43 ± 0.06
2.55 ± 0.06
3.21 ± 0.14
2.52 ± 0.07
2.68 ± 0.07
2.94 ± 0.12
3.57 ± 0.15
3.83 ± 0.18
3.77 ± 0.23
3.55 ± 0.15
3.77 ± 0.18
3.58 ± 0.22
0.31 ± 0.02
0.84 ± 0.06
0.41 ± 0.03
0.83 ± 0.06
0.13 ± 0.01
0.33 ± 0.01
0.19 ± 0.01
0.24 ± 0.02
1.03 ± 0.04
1.27 ± 0.05
1.60 ± 0.14
1.03 ± 0.04
1.34 ± 0.06
1.63 ± 0.13
Global analysis of intracellular amino acid pools
Overall, these data reflect a higher impact of oxygen availability rather than recombinant protein production on the global physiologic response of P. pastoris, consistent with previous transcriptomic, proteomic and metabolic flux analyses. To help decipher the potential dependence of the amino acid metabolism on oxygen availability and the burden caused by recombinant protein secretion, a more detailed analysis was performed focusing on each of these two factors separately.
Recombinant protein production effect
The amino acid pools provide building blocks for protein synthesis. Therefore, an impact of Fab production on these metabolites was a priori expected. In fact, previous studies using amino acids for media supplementation or complex extracts have proven to have a positive effect on recombinant protein production[22, 27, 33].
The impact of recombinant protein production on amino acid pool sizes under normoxic conditions is shown in Figure 2A. Overall, 10 out of 17 amino acid pools increased their intracellular levels significantly, while only 2 of them had an opposite behavior. In particular, most of the free amino acids from the Ala, Asp and Phe families displayed significantly increased levels in the Fab-expressing strain. Nevertheless, the Glu family showed different trends depending on the amino acid, being Orn and Pro significantly decreased, while Gln increased. Increased amino acid pools in the Fab-expressing strain were not accompanied by any significant flux redistribution though the central metabolism nor proteome compositional change, when compared to the control strain growing under the same oxygenation condition[30, 31].
Conversely, generally decreased amino acid pool ratios (11 out of 17) were found in the Fab-expressing strains under oxygen-limiting conditions, compared to the reference strain (Figure 2B), that is, an opposite behavior to that observed under normoxic conditions. We observed the same trend when comparing the Fab-expressing strain growing under hypoxic condition; however, the data variance was higher and, therefore, the observed changes were not statistically significant. A priori, this observation might be associated to the increased specific heterologous protein productivity found under oxygen limitation, as the Fab producing strain might require higher metabolic fluxes of amino acids for protein synthesis, thereby causing a drain of precursor pools. Nevertheless, this hypothesis is highly unlikely, since Fab production levels were relatively low in relation to the total cell protein. Besides, this trend was not uniform within each of the amino acid families. For instance, in the Ala family, Ala and Leu pools increased while Val decreased in the Fab-expressing strain. Also, in the Asp amino acid family only Asp levels varied significantly. Moreover, no correlation was found between the relative abundance of each amino acid in the Fab antibody fragment and the observed changes in the corresponding free amino acid pools (Figure 2D). Nevertheless the specific Fab production correlated inversely (correlation value of −0.93) with the average of the metabolite ratios between Fab-expressing and reference strains at each culture condition. This may reflect a global re-adjustment of the free amino acid pools to compensate for the recombinant protein overproduction. Although such readjustment could not be the result from a direct drain of building blocks at higher Fab synthesis levels, other phenomena related with recombinant protein production might provide some explanations. For instance, recent studies on recombinant protein secretion using 34 S labeling strategies with the same strain used in this study have revealed that about 58% of the Fab protein produced intracellularly is actually degraded within the cell, and only 35% is secreted. In addition to protein degradation, increased energetic demands related with the cost of the folding, refolding and secretion processes of the Fab product could result in an overall readjustment of amino acid metabolism. In fact, when the Fab producing strain was cultivated at lower temperatures (20°C), the unfolded protein stress response was reduced, leading to a reduced metabolic burden and higher specific productivities compared with cells grown at 25°C and 30°C, in which an increase in energy demand was evidenced by an up-regulation of the TCA cycle, slightly higher in Fab-secreting strains. Besides, increased maintenance requirements associated with heterologous protein production (e.g. unfolded protein stress response) may cause additional energy demands. Interestingly, recent amino acid supplementation studies using a recombinant P. pastoris strain revealed that such supplementation partially relieved the metabolic burden from recombinant protein production. Furthermore, de novo amino acid synthesis in cells growing on different combinations of amino acids supplementations was inversely correlated with the corresponding energetic cost for most amino acids.
Oxygen availability effect
Previous transcriptomic studies revealed amino acid metabolism as one of the major cellular processes regulated by oxygen availability. Interestingly, the number of genes in the gene ontology group of amino acid metabolism that were down regulated under hypoxic conditions was substantially higher in the Fab-producing strain.
Overall, changes in free amino acid pools seemed to correlate directly with changes in the relative abundance in the cell proteome for most amino acids, suggesting that biosynthetic fluxes to cell protein had a direct impact on the precursor amino acids pools sizes. A clear exception to this observation was Ala and, to a much lesser extent, Asx (Asp + Asn). Unfortunately, the available metabolomic methodology did not allow for accurate quantification of the metabolite precursors of these amino acids (that is, Pyr and OAA), hampering the interpretation of the observed changes in Ala and Asx pool sizes.
A direct comparison of the relative changes observed in the free amino acid levels between different oxygen availability conditions and the corresponding changes observed in the whole cell’s amino acid component (that is, free intracellular amino acid pools plus cell protein amino acids) indicated that the latter were less pronounced.
The integrated data seem to reflect the oxygen-dependent transcriptional regulation of amino acid biosynthesis pathways. In particular, transcriptional levels of several key regulatory enzymes in the biosynthetic pathways correlated inversely with the carbon flow through them, as well as with their corresponding end-metabolite levels, probably denoting the negative feedback control of the pathway. For instance, the levels of free tyrosine and phenylalanine were substantially increased under oxygen-limiting conditions, concomitantly with a reduction of the mRNA levels of ARO7, while an opposite pattern was observed for the free tryptophan pools and TRP3 transcriptional levels (Figure 5). This pattern was also observed in other amino acid biosynthetic pathways such as methionine (see Additional file2).
Similarly, in the glutamate amino acid family (Figure 6), the carbon flow to lysine was increased under oxygen limiting conditions, concomitantly with a reduction in transcript levels of several genes of its pathway, particularly LYS20, the first reaction of the pathway. In addition, the carbon flux to the Glu and Gln biosynthetic pathway was significantly reduced at lower oxygen availability. This pattern correlated directly with the reduced transcriptional levels of GDH3 and GLN1.
Conversely, the reduced Ala and Asp biosynthetic fluxes to cell protein under limited oxygen levels seemed to be directly correlated to ATL1 and ATT1/ATT2 transcriptional levels, respectively (Additional files2 and3). Also, no significant variation on the His biosynthetic flux was observed despite the significant transcriptional and His pool sizes changes measured upon oxygen limitation.
Previous transcriptomic studies pointed at the impact of oxygen availability and recombinant protein production on amino acid metabolism. The analysis at the metabolomic level further confirms such impact. In particular, our data point at a major impact of oxygen availability rather than recombinant protein production on the free amino acid pools, coherent with previous transcriptional analyses. Notably, changes in free amino acid pools observed at different oxygenation conditions generally correlated directly with the changes in relative abundances in the corresponding amino acids of the cell’s proteome, with alanine being the major exception. In contrast, the impact of recombinant protein production on the free amino acids pools depended on the oxygenation state. Importantly, the observed changes did not correlate with the difference in amino acid composition of the recombinant product and the cell’s proteome, but rather to the energetic costs (specifically, for those amino acids with highest energy costs), thereby suggesting a possible dependence between mitochondrial metabolism and amino acid anabolism as a potential target to modulate the metabolic burden caused by recombinant protein production.
Systematic integration of metabolomic and transcriptional data into genomic-scale metabolic models should allow gaining further understanding of the behaviour of central and amino acid metabolism, as well as identifying metabolic bottlenecks limiting enhanced recombinant protein production.
Strain and cultivation conditions
Analytical grade reagents were supplied by Sigma-Aldrich. HPLC-grade methanol and ethanol were supplied by J.T. Baker.
In this study, the P. pastoris strain X-33 pGAPZαA Fab3H6, secreting the light and heavy chains of a human monoclonal antibody Fab fragment under the constitutive GAP promoter and the S. cerevisiae alpha-mating factor leader, was used as expressing strain. A strain with an integrated empty-vector was used as reference strain. The experimental set up was as described in. Briefly, glucose-limited chemostat cultures at a dilution rate of 0.1 h-1 at different oxygenation conditions were carried out by changing the oxygen content of the inlet gas. Initially, the oxygen concentration in the inlet gas stream corresponded to normal air (20.95% v/v) leading to a totally normoxic condition (i.e. pO2 > 20%, fully respiratory metabolism). Inlet gas oxygen levels were subsequently stepwise reduced by replacing different air proportions with nitrogen. Thereby either oxygen limited or hypoxic conditions were created in the bioreactor which are characterized by different ethanol and arabinitol production rates[29–31]. Two chemostats were performed for each metabolic steady state and strain.
The different chemostat conditions were maintained for 5 residence times before sampling. As previously reported, this cultivation time is enough to reach a metabolic steady state in P. pastoris. For each steady state condition, duplicate samples for intracellular metabolite measurement were taken using the previously described optimized protocol for the direct measurement of P. pastoris metabolome. For cellular dry weight, a known volume of cultivation broth was filtered using pre-weighted filters; these were washed with two volumes of distilled water and dried to constant weight at 70°C.
Samples for extracellular metabolite were obtained by rapid sampling of broth with immediate cooling to 0°C and fast filtration, using the cold steel-bead method and analyzed using high-performance liquid chromatography (HPLC) with a Bio-Rad Aminex column at a temperature of 60°C. The mobile phase was 5.0 mM phosphoric acid of which the flow rate was set to 0.6 mL/min.
Free intracellular amino acid analysis
The intracellular concentrations of Ala, Val, Leu, Ile, Thr, Asn, Asp, Met, Trp, His, Pro, Glu, Gln, Orn, Lys, Tyr and Phe were determined by GC-MS. Briefly, 100 μL of sample were transferred to a glass vial, 30 μL of 100 mg/mL NaCl were added and the mixture was lyophilized. 75 μL acetonitrile and 75 μL of N-methyl-N-(tert- butyldimethylsilyl)trifluoroacetamide (MTBSTFA, Thermo Scientific) were added and the vial was incubated for 1 h at 70°C. Subsequently, the sample was centrifuged (10,000 g, 2 min) and 60 μL of the supernatant were transferred to a GC glass vial with an insert. The sample was then analyzed by GC–MS instrument coupled to a 5975 C MSD single quadrupole mass spectrometer (Agilent, Santa Clara, CA, USA). Glycine levels were also measured in this analysis; however, the obtained results were not included as the values were found to be inconsistent. Quantification was based on isotope dilution mass spectrometry (IDMS). In total, 17 amino acids were analyzed plus Glycine.
In order to graphically visualize the obtained intracellular quantification together with the central carbon metabolism and amino acid biosynthetic pathways the VANTED software was used.
Antibody fragment quantification
Fab amounts in soluble cell extracts and in culture broths were performed by means of a sandwich ELISA assay as previously described.
Principal component analysis
Reference values for each amino acid pool level were calculated as mean of all measurements independently of the strain or oxygen availability. Afterwards, for each condition, the specific amino acid measurements were divided by the reference value to obtain a free scale value of its variation. These relative fold changes for each condition were illustrated through PCA using the xlstat plug-in software for Excel.
Determination of energy and degree of reduction for amino acid synthesis
The degree of reduction of each amino acid were calculated using the amino acid molecular formulas as explained elsewhere. Energy costs for the biosynthesis of each amino acid were taken from previously published data for S. cerevisiae growing under aerobic conditions as both yeast share equal amino acid biosynthetic pathways[7, 8, 11].
This work has been supported by the Spanish program on Chemical Process Technologies (project CTQ2010-15131) of the Spanish Ministry of Science and Innovation, the Generalitat de Catalunya (Contract Grant 2009-SGR-281 and Xarxa de Referència en Biotecnologia) and the Spanish Ministry scholarship TME2009-00591.
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