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Table 1 Summary of macromolecular culture parameters

From: Quantitative metabolomics analysis of amino acid metabolism in recombinant Pichia pastoris under different oxygen availability conditions

  

Control Strain

  

Expressing Strain

 

Normoxic

O2-limited

Hypoxic

Normoxic

O2-limited

Hypoxic

qFab

   

40 ± 5

82 ± 2

74 ± 9

qGlc

−1.00 ± 0.02

−1.28 ± 0.03

−1.72 ± 0.05

−1.01 ± 0.02

−1.37 ± 0.03

−1.56 ± 0.04

qO2

−2.35 ± 0.06

−2.01 ± 0.07

−2.01 ± 0.15

−2.44 ± 0.07

−1.99 ± 0.08

−1.81 ± 0.13

qCO2

2.43 ± 0.06

2.55 ± 0.06

3.21 ± 0.14

2.52 ± 0.07

2.68 ± 0.07

2.94 ± 0.12

qX

3.57 ± 0.15

3.83 ± 0.18

3.77 ± 0.23

3.55 ± 0.15

3.77 ± 0.18

3.58 ± 0.22

qEtOH

 

0.31 ± 0.02

0.84 ± 0.06

 

0.41 ± 0.03

0.83 ± 0.06

qAra

 

0.13 ± 0.01

0.33 ± 0.01

 

0.19 ± 0.01

0.24 ± 0.02

RQ

1.03 ± 0.04

1.27 ± 0.05

1.60 ± 0.14

1.03 ± 0.04

1.34 ± 0.06

1.63 ± 0.13

  1. qGluc and qO2 are specific utilization rates, and qX, qAra, qEtOH and qCO2 are specific production rates, where Glc, Ara, EtOH and X stand for glucose, arabinitol, ethanol and biomass, respectively. Conversion rates are given in mmol/(gDCW·h), except for qFab, which is given in μgFab/(gDCW·h) . DCW, dry cell weight. RQ = qCO2 / qO2.