Hyperproduction of poly(4-hydroxybutyrate) from glucose by recombinant Escherichia coli
- Xiao-Yun Zhou†1,
- Xiao-Xi Yuan†1,
- Zhen-Yu Shi2,
- De-Chuang Meng1,
- Wen-Jun Jiang1,
- Lin-Ping Wu3,
- Jin-Chun Chen1Email author and
- Guo-Qiang Chen1, 4Email author
© Zhou et al; licensee BioMed Central Ltd. 2012
Received: 31 March 2012
Accepted: 26 April 2012
Published: 2 May 2012
Poly(4-hydroxybutyrate) [poly(4HB)] is a strong thermoplastic biomaterial with remarkable mechanical properties, biocompatibility and biodegradability. However, it is generally synthesized when 4-hydroxybutyrate (4HB) structurally related substrates such as γ-butyrolactone, 4-hydroxybutyrate or 1,4-butanediol (1,4-BD) are provided as precursor which are much more expensive than glucose. At present, high production cost is a big obstacle for large scale production of poly(4HB).
Recombinant Escherichia coli strain was constructed to achieve hyperproduction of poly(4-hydroxybutyrate) [poly(4HB)] using glucose as a sole carbon source. An engineering pathway was established in E. coli containing genes encoding succinate degradation of Clostridium kluyveri and PHB synthase of Ralstonia eutropha. Native succinate semialdehyde dehydrogenase genes sad and gabD in E. coli were both inactivated to enhance the carbon flux to poly(4HB) biosynthesis. Four PHA binding proteins (PhaP or phasins) including PhaP1, PhaP2, PhaP3 and PhaP4 from R. eutropha were heterologously expressed in the recombinant E. coli, respectively, leading to different levels of improvement in poly(4HB) production. Among them PhaP1 exhibited the highest capability for enhanced polymer synthesis. The recombinant E. coli produced 5.5 g L-1 cell dry weight containing 35.4% poly(4HB) using glucose as a sole carbon source in a 48 h shake flask growth. In a 6-L fermentor study, 11.5 g L-1 cell dry weight containing 68.2% poly(4HB) was obtained after 52 h of cultivation. This was the highest poly(4HB) yield using glucose as a sole carbon source reported so far. Poly(4HB) was structurally confirmed by gas chromatographic (GC) as well as 1H and 13C NMR studies.
Significant level of poly(4HB) biosynthesis from glucose can be achieved in sad and gabD genes deficient strain of E. coli JM109 harboring an engineering pathway encoding succinate degradation genes and PHB synthase gene, together with expression of four PHA binding proteins PhaP or phasins, respectively. Over 68% poly(4HB) was produced in a fed-batch fermentation process, demonstrating the feasibility for enhanced poly(4HB) production using the recombinant strain for future cost effective commercial development.
A large variety of bacteria are able to accumulate diverse polyhydroxyalkanoates (PHA) as intracellular carbon and energy storage material under nutritional unbalanced conditions [1–4]. Due to their diverse structures, chirality, biodegradability and biocompatibility, PHA have attracted attentions from academic and industrial communities for their potential applications in areas of agriculture, medicine, and materials [2, 5–7]. More than 150 types of hydroxyalkanoic acids have been known as monomers of PHA, leading to diverse polymer physical properties [8–11]. Some of the PHA monomers and oligomers were reported to stimulate cell proliferations [12, 13].
Homopolyesters of 4-hydroxybutyrate, or Poly(4HB), is a strong thermoplastic material with an elongation to break of 1000%, which means it can be stretched 10 times its original length before it is broken . Due to the remarkable mechanical properties, biocompatibility and biodegradability, poly(4HB) has been approved by the United States Food and Drug Administration (FDA) as the first PHA medical implant material among several PHA materials under investigation [5, 15, 16].
4-hydroxybutyrate (4HB) was first detected in a copolyester of 3HB and 4HB isolated from R. eutropha and generally the incorporation of 4HB into PHA occurred only if 4-Hydroxybutyrate, 4-butyrolactone, 1,4-butanediol or 4-chlorobutyrate was provided as carbon source . Poly(4HB) homopolyester was first reported to be synthesized by R. eutropha using 4-hydroxybutyrate as a precursor . Wild-type bacteria like Comanonas acidovorans and Hydrogenophaga pseudovorans were also found to produce poly(4HB). Recombinant E. coli expressing R. eutropha PHA synthase gene phaC and Clostridium kluyveri 4HB-CoA:CoA transferase gene orfZ were found able to synthesize poly(4HB) homopolyester when both glucose and 4HB were provided as carbon sources [22, 23]. As a general rule, structurally related substrates of 4HB such as γ-butyrolactone, 4-hydroxybutyrate or 1,4-butanediol (1,4-BD) are required as precursors for poly(4HB) synthesis . However, these substrates are much more expensive than glucose, leading to the high cost of poly(4HB) production. Song et al. succeeded in producing poly(4HB) homopolyester using glucose as a sole carbon source in recombinant E. coli. However, the reported yield (0.78 g L-1) was low for mass cultivation. The high cost of raw material and the low yield of poly(4HB) prevents wide exploitation of poly(4HB) for more applications .
E. coli possesses two forms of SSA dehydrogenase (SSADH) encoded by sad and gabD first identified in R. eutropha, both SSADH catalyze degradation of SSA to succinate which can reduce the SSA flux to 4HB-CoA pathway. Li et al. reported an enhancement on 4HB content in P3HB4HB when SSADH was inactivated in E. coli, indicating a possibility of producing poly(4HB) in sad and gabD deficient mutant.
Phasins are small amphiphilic proteins localizing at the surface of PHA granules and there are interactions among various phasins [32–35]. They play important roles in PHA synthesis and granule formation . The PhaP phasins were proven to promote PHB synthesis by regulating the surface/volume ratio of PHB granules or by interacting with PHA synthase yet without influencing PHA molecular weights . Four genes encoding highly homologous phasins including phaP1, phaP2, phaP3 and phaP4 were found in R. eutropha, among which PhaP1 is the major phasin protein [38–40].
The aim of this study was to construct a recombinant E. coli for hyperproduction of poly(4HB) using glucose as a sole carbon source.
Synthesis of poly(4HB) by recombinant E. coli grown in shake flasks
Shake flasks study of poly(4HB) production from glucose by E. coli strains grown in shake flasks a
CDW b (g L-1)
Poly(4HB) content c (wt%)
Poly(4HB) (g L-1)
Production and structure confirmation of poly(4HB) from fermentor study
Physical characterization of poly(4HB) produced by the recombinant E. coli
Poly(4HB) produced by E. coli JM109SG (pKSSEP1, pMCSH5) was extracted and purified before casting into films for mechanical property studies. The white and foggy poly(4HB) films exhibited a much higher elasticity than other known PHA. Its elongation at break (εb), tensile strength (σt), and Young’s modulus (E) was 1014%, 32.55 MPa and 12.8 MPa, respectively. The poly(4HB) had a molecular mass of weight average molecular weigh (Mw) of 0.22 × 106 together with a polydispersity (Mw/Mn) of 2.11 as determined by gel-permeation chromatography.
As a strong pliable thermoplastic material with good flexibility, poly(4HB) has been approved by FDA as a suture material (http://www.tepha.com). Biomedical applications are usually not sensitive to high cost. However, a reduction on poly(4HB) production cost should allow for more application exploitation. High production cost for poly(4HB) comes from expensive 4HB precursors including 4-hydroxybutyric acid, γ-butyrolacton or 1,4-butanediol [41, 42], and from very low yield of poly(4HB) by recombinant bacteria. Therefore, simple and low cost substrates as well as a highly productive strain can help reduce poly(4HB) production cost.
The anaerobic succinate degradation pathway employed in this study conferred on the recombinant E. coli the ability to utilize glucose as a sole carbon source for poly(4HB) production. The additional expression of PHA granule associate protein PhaP provided a further enhancement on poly(4HB) yield, allowing for further fermentor exploitation. While in wild E. coli strain, succinate semialdehyde can be degraded to succinate by SSA dehydrogenase (SSADH) encoded by sad and gabD, leading to a decreased metabolic flux to 4HB production (Figure 1). To channel more flux to 4HB, the native SSADH genes of E. coli were inactivated in the poly(4HB) producing recombinant. Shake flask studies of E. coli JM109 and its SSADH deficient mutant JM109SG harboring pMCSH5 and pKSSE5.3 showed that inactivation of SSADH genes significantly improved poly(4HB) synthesis compared with the wild strain JM109 which had no poly(4HB) production at all (Table 1). On the other hand, the highest 4HB molar fraction in P3HB4HB synthesized from glucose in E. coli was 11% reported so far . Our result indicated that the recombinant enzymes in this pathway were active enough to provide sufficient 4HB from glucose for polymerization.
Expression of all four PhaPs (phasin) cloned from R. eutropha provided additional improvement on poly(4HB) accumulation in the order of PhaP1 > PhaP3 > PhaP2 > PhaP4 (Table 1). The differences of their different influences are not clear yet but probably due to the different roles of PhaP played on PHA granules formation. PhaP1 was the major phasin with the highest expression amount in R. eutropha while PhaP2, PhaP3 and PhaP4 were small in quantity [39, 40], indicating its dominating function for PHA granule formation, and PhaP3 was expressed at a significantly high level in PhaP1 deficient strains, other PhaPs were in much lower levels. Our results therefore suggested that the poly(4HB) yields were positively related to the expression levels of PhaP.
The recombinant E. coli JM109SG (pKSSEP1, pMCSH5) grown to 12 g L-1 CDW under a well-controlled fermentor run in a fed-batch process accumulated over 68% poly(4HB) using glucose as the only carbon source over a 52 h period (Figure 3). This is by far the highest yield for poly(4HB). In its exponential growth period of 8–24 h after innoculation, poly(4HB) content increased most rapidly and reached a relatively stable level when cells entered the stationary phase. As in the exponential phase, TCA cycle is most active, supplying the most succinyl-CoA for the poly(4HB) synthesis, leading to a rapid poly(4HB) accumulation rate. A continuous fermentation process that maintains the cells in their exponential growth phase may further improve poly(4HB) accumulation level.
In summary, Escherichia coli strain JM109 harboring an engineering pathway encoding succinate degradation genes of Clostridium kluyveri and PHB synthase gene of Ralstonia eutropha together with its native succinate semialdehyde dehydrogenase genes sad and gabD inactivated, was able to achieve significant level of poly(4HB) biosynthesis from glucose. Additional expression of four PHA binding proteins PhaP or phasins in the recombinant strain, respectively, led to a further improvement of poly(4HB) accumulation. PhaP1 was found most useful among the four PhaPs used. Over 68 wt% poly(4HB) was produced in a fed-batch fermentation process, demonstrating the feasibility for enhanced poly(4HB) production using the recombinant strain for future cost effective commercial development.
Bacterial strains and plasmids
Bacterial strains and plasmids used in this study
Source or reference
E. coli JM109
recA1, endA1, gyrA96, thi, hsdR17, supE44, relA1, Δ(lac proAB)/F’
TaKaRa (Dalian, China)
[traD36, proAB + , lac q lacZΔ M15]
E. coli JM109SG
JM109 ∆sad ∆gabD
E. coli Trans1-T1
The fastest growing chemically competent strain currently available
TransGen Biotech (Beijing, China)
Ralstonia eutropha H16
pBluescript vector derived, containing phaC and orfZ, AmpR
phaP1 gene inserted into pKSSE5.3, AmpR
phaP2 gene inserted into pKSSE5.3, AmpR
phaP3 gene inserted into pKSSE5.3, AmpR
phaP4 gene inserted into pKSSE5.3, AmpR
sucD-4hbD inserted into pBBR1MCS-2, KmR
Primers (5 ′ → 3′)
Plasmid pKSSE5.3 was constructed by Hein et al. containing genes phaC and orfZ. Plasmids pKSSEPx, with “x” referring to 1, 2, 3 and 4 in this study, were constructed by subcloning genes phaP1, phaP2, phaP3 and phaP4 from genome of R. eutropha, followed by inserting them into the plasmid pKSSE5.3, respectively. Primers phaPxF and phaPxR were used for PCR amplification. The PCR products of phaP1 phaP2 and phaP3 fragments were digested by StuI while phaP4 fragment digested by pFlfI. The gel electrophoresis-purified fragments were then ligated with pKSSE5.3 digested by the respective endonuclease (Figure 2). Plasmid pMCSH5 was constructed by Li et al. containing sucD and 4hbD genes .
Cultivation conditions and culture medium
Plasmids pKSSE5.3 or pKSSEPx were co-transformed with pMCSH5 into E. coli JM109 and its SSADH deficient mutant by electroporation.
For shake flask cultivations, the growth process was carried out on a rotary shaker at 200 rpm in 500 mL conical flasks containing 50 mL LB medium supplemented with 20 g L-1 glucose at an inoculation volume of 4% for 48 h. For fermentation studies, seed culture was inoculated into a 6-L fermentor (NBS3000, New Brunswick, USA) at 10% inoculation volume with an operating volume of 3 L. The fermentation process was carried out at 37°C, pH 7.0 under a dissolved oxygen concentration (DO) of 30% of saturation. For poly(4HB) accumulation, concentrated glucose was complemented when OD600 began to increase exponentially to maintain the concentration of glucose above 10 g L-1 during the fermentation process.
For shake flask studies, Luria-Bertani (LB) medium supplemented with 20 g L-1 glucose in phosphate buffered saline (PBS) solution with a working concentration of 2.31 g L-1 KH2PO4 and 16.42 g L-1 K2HPO4∙3H2O to maintain a pH around 7.0. Glucose and PBS were added to the medium after heat sterilization. During the fermentation process, LB medium with 15 g L-1 yeast extract without PBS was used to promote cell growth. 50 mg L-1 kanamycin and 100 mg L-1 ampicillin were added to the medium to maintain stability of the plasmids during the growth processes.
Bacterial cultures were harvested by centrifugation at 3000 g for 10 min and then washed with distilled water. The cell dry weight (CDW) was measured after vacuum lyophilization. PHA content and composition were analyzed by gas chromatography (SHIMADZU GC-2014 C, Kyoto, Japan) after methanolysis of lyophilized cells in chloroform with γ-butyrolactone (Sigma-Aldrich) used as standard [44, 45].
PHA extraction and physical characterization
PHA were extracted from the lyophilized cells . In details: 10 mL chloroform was added to 1 g of dry cells in screw-capped tubes. The tubes were maintained at 100°C for 4 h. Subsequently, equal volume of water was added to the tube and the chloroform at the bottom was sucked out and precipitated with an excess of 10 volumes of ice-cold ethanol .
The molecular structure of poly(4HB) was studied using nuclear magnetic resonance (NMR). The sample was dissolve in deuterated-chloroform (CDCl3) and recorded the 1H NMR and 13C NMR spectra. The proton (1H) NMR was performed on JOEL JNM- ECA 300 NMR spectrophotometer in deuterated chloroform as a solvent, tetramethylsilane (TMS) was used as an internal chemical shift standard. Carbon (13C) NMR spectra was measured on 600 MHz spectrophotometer.
To study its mechanical properties, PHA samples were spread into films by the conventional solvent-casting method . The resulting PHA films were cut into rectangle-shaped specimens with a width of 10 mm and a thickness of approximately 120 μm. The stress–strain measurements of films were carried out using an AL-7000 S testing machine (Gotech Testing Machine, China) at room temperature. The speed of the cross-head was 50 mm min-1. Molecular weights were obtained via gel permeation chromatography (GPC Spectra System P2000) equipped with a Shimadzu RID-10A detector.
We are grateful to Professor Alexander Steinbüchel of the University of Münster in Germany for the generous donation of Ralstonia eutropha H16. We are also very grateful to Professor Shui-Shan Song of Biology Institute of Hebei Province in Shijiazhuang/China for the kind donation of plasmid pKSSE5.3. This research was supported by the State Basic Science Foundation 973 (Grant No. 2012CB725200, 2012CB725201 and 2011CBA00807). Grants from National Natural Science Foundation of China (Grant No. 31170099 and 30570024) also contributed to this project.
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