Hyperproduction of poly(4-hydroxybutyrate) from glucose by recombinant Escherichia coli

Table 1 Shake flasks study of poly(4HB) production from glucose by E. coli strains grown in shake flasks ^a

E. coli	Plasmids	CDW^b(g L^-1)	Poly(4HB) content^c (wt%)	Poly(4HB) (g L^-1)
JM109	pKSSE5.3, pMCSH5	3.33±0.13	-^d	-
JM109SG	pKSSE5.3, pMCSH5	3.83±0.15	12.13±0.53	0.47±0.04
JM109SG	pKSSEP1, pMCSH5	5.46±0.04	35.39±0.80	1.93±0.06
JM109SG	pKSSEP2, pMCSH5	4.30±0.17	22.85±1.06	0.98±0.07
JM109SG	pKSSEP3, pMCSH5	4.28±0.12	32.35±1.42	1.39±0.09
JM109SG	pKSSEP4, pMCSH5	4.34±0.15	22.95±2.58	1.00±0.14

^a Cells were cultivated in LB medium at 37°C and 200 rpm for 48 h as described in “Materials and methods”
20 g L^-1 glucose and PBS buffer were added to the medium after sterilization. Three parallel studies were conducted for each data.
^b CDW: cell dry weight.
^c Poly(4HB) content: PHA contents are given as mass percentage of CDW.
^d Not detected.

ISSN: 1475-2859