Plasmids/strains | Modules/genotype | References |
---|---|---|
Plasmids | ||
BB1_12_pAOX1 | PAOX1, KanR+ | [5] |
BB1_12_pFDH1 | PFDH1, KanR+ | |
BB1_12_pPOR1 | PPOR1, KanR+ | |
BB1_12_pPDC1 | PPDC1, KanR+ | |
BB1_23 | Ø, KanR+ | |
BB1_34_RPS3tt | RPS3tt, KanR+ | |
BB1_34_ScCYC1tt | ScCYC1tt, KanR+ | |
BB1_34_TDH3tt | TDH3tt, KanR+ | |
BB2_BC | Ø, AmpR+ | |
pCC1-4k_TcPAND | panD+, T. castaneum gene encoding for PANDTc enzyme | GenScript Biotech |
pCC1-4k_BcBAPAT | yhxA+, B. cereus gene encoding for BAPATBc | |
BB1_23_EcYDFG | ydfG+, E. coli str. K-12 gene encoding for YDFGEc | |
BB1_23_PseFDH(V9) | fdh+, Pseudomonas sp. (strain 101) gene encoding for FDHPse(V9)(A199G/D222Q/S381V/C256A/H380K) | |
BB2_BC_TcPAND | PAOX1-PANDTc-RPS3tt | This work |
BB2_BC_BcBAPAT | PFDH1-BAPATBc-ScCYC1tt | |
BB2_BC_EcYDFG | PPOR1-YDFGEc-TDH3tt | |
PPDC1-YDFGEc-TDH3tt | ||
BB2_BC_PseFDH(V9) | PFDH1-FDH(V9)Pse-TDH3tt | |
BB3nK_ext_AD | Ø, KanR+ | [6] |
BB3nK_ext_AD_TcPAND | 5′-HR_PAOX1-PANDTc-RPS3tt_3′-HR(RGI2) | This work |
5′-HR_PAOX1-PANDTc-RPS3tt_3′-HR(pGAPUP) | ||
BB3nK_ext_AD_BcBAPAT | 5′-HR_PFDH1-BAPATBc-ScCYC1tt_3′-HR(pTEF1-αUP) | |
BB3nK_ext_AD_EcYDFG | 5′-HR_PPOR1-YDFGEc-TDH3tt_3′-HR(pFLD1UP) | |
5′-HR_PPDC1-YDFGEc-TDH3tt_3′-HR(pFLD1UP) | ||
BB3nK_ext_AD_PseFDH(V9) | 5′-HR_PFDH1-FDH(V9)Pse-TDH3tt_3′-HR(pAOX1UP) | |
BB3cN_pGAP_23*_pLAT1_Cas9 | PGAP_Ø_PLAT1_Cas9, NrsR+ | [6] |
BB3cK_pGAP_23*_pLAT1_Cas9 | PGAP_Ø_PLAT1_Cas9, KanR+ | |
BB3cH_pGAP_23*_pLAT1_Cas9 | PGAP_Ø_PLAT1_Cas9, HygR+ | |
BB3cK_pGAP_gRNA1(RGI2)_pLAT1_Cas9 | PGAP_gRNA1(RGI2)_PLAT1_Cas9, KanR+ | This work |
BB3cH_pGAP_gRNA1(pGAPUP)_pLAT1_Cas9 | PGAP_gRNA1(PGAPUP)_PLAT1_Cas9, HygR+ | |
BB3cK_pGAP_gRNA2(pTEF1-αUP)_pLAT1_Cas9 | PGAP_gRNA2(PTEF1-αUP)_PLAT1_Cas9, KanR+ | |
BB3cN_pGAP_gRNA1(pFLD1UP)_pLAT1_Cas9 | PGAP_gRNA1(PFLD1UP)_PLAT1_Cas9, NrsR+ | |
BB3cN_pGAP_gRNA1(pAOX1UP)_pLAT1_Cas9 | PGAP_gRNA1(PAOX1UP)_PLAT1_Cas9, NrsR+ | |
K. phaffii strains | ||
CBS7435 | Wild-type | CBS, Centraalbureau voor Schimmelcultures |
PpCβ10 | PAOX1_PANDTc + PFDH1_BAPATBc + PPOR1_YDFGEc | This work |
PpCβ20 | PAOX1_PANDTc + PFDH1_BAPATBc + PPDC1_YDFGEc | |
PpCβ11 | 2x(PAOX1_PANDTc) + PFDH1_BAPATBc + PPOR1_YDFGEc | |
PpCβ21 | 2x(PAOX1_PANDTc) + PFDH1_BAPATBc + PPDC1_YDFGEc | |
PpCβ20-P | PpCβ20 + PFDH1_FDH(V9)Pse | |
PpCβ21-P | PpCβ21 + PFDH1_FDH(V9)Pse |