Fig. 5

RT-qPCR analysis of the three secretory pathway component genes in the cbh1-integrated and cel3c-integrated T. reesei strains. A: Analysis of the transcription for samples taken at 24 h post induction; B: Analysis of the transcription for samples taken at 48 h post induction. Expression of AnGOx was induced with cellulose in these T. reesei strains. At 24 h and 48 h post induction, mycelia were collected. The total RNA was extracted from the mycelia and then reverse-transcribed to cDNA. In RT-qPCR analysis, the sar1 gene was used as the internal reference gene. For both the 2 time points and all three genes, the transcript abundance of the analyzed gene in the QM9414 was set as 1.0. The relative transcript level of the gene in QM9414-cbh1 and QM9414-cel3c were calculated through dividing by that in the QM9414 strain. ns not significant; *, 0.01 < P < 0.05; ****, P < 0.0001