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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Combining manipulation of integration loci and secretory pathway on expression of an Aspergillus niger glucose oxidase gene in Trichoderma reesei

Fig. 4

Effect of overexpressing three component genes in the secretion pathway on the secretory production of AnGOx in cbh1-integrated and cel3c-integrated strains. A-C: The plasmid maps for pPpdc1-snc1 (A), pPpdc1-sso2 (B) and pPpdc1-rho3 (C). D: The glucose oxidase activities in QM9414-cbh1, QM9414-cbh1-snc1, QM9414-cbh1-sso2, QM9414-cbh1-rho3, QM9414-cel3c, QM9414-cel3c-snc1, QM9414-cel3c-sso2, and QM9414-cel3c-rho3 on day 6 post induction. E and F: The relative transcript levels of snc1 in QM9414-cbh1-snc1 (QM9414-cbh1 overexpressing the secretory component gene snc1), sso2 in QM9414-cbh1-sso2, rho3 in QM9414-cbh1-rho3, snc1 in QM9414-cel3c-snc1 (QM9414-cel3c overexpressing the secretory component gene snc1), sso2 in QM9414-cel3c-sso2, and rho3 in QM9414-cel3c-rho3 at 24 h (E) and 48 h (F) post cellulose induction. The transcript level of the corresponding genes in QM9414-cbh1 or QM9414-cel3c was set as the reference with a value of 1.0. The relative transcript levels of the genes in the other strains were calculated through dividing by the corresponding genes in the QM9414-cbh1 or QM9414-cel3c strains. *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, P < 0.001

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Microbial Cell Factories

ISSN: 1475-2859

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