Fig. 3

AnGOx was expressed at a higher level in the early stage of induction in QM9414-cel3c transformant as analyzed by RT-qPCR. A and B: comparison of the AnGOx expression in QM9414-cbh1 and QM9414-cel3c strains at 24 h (A) and 48 h B. The T. reesei cells were induced with cellulose. At 24 h and 48 h post induction, mycelia were collected. The total RNA was extracted from the mycelia and then reverse-transcribed to cDNA. In RT-qPCR analysis, the sar1 gene was used as the internal reference gene. For both the 2 time points, the transcript abundance of AnGOx in the QM9414-cbh1 was set as 1.0. The relative transcript level of AnGOx in the QM9414-cel3c was calculated through dividing by that in the QM9414-cbh1 strain. ns not significant; ****, P < 0.0001