Table 2 Metabolic engineering application in filamentous yeast
From: Microbial cell factories based on filamentous bacteria, yeasts, and fungi
Category | Strategies | Strains | Products | Yield (g/g) | Titer (g/L) | Productivity (g/L/h) | Refs. |
|---|---|---|---|---|---|---|---|
Filamentous yeast | C. glabrata | T. glabrata CCTCC M202019 | Pyruvate | 0.44 | 40.2 | 0.56 | [55] |
T. glabrata T.G-PMS | L-malate | – | 8.5 | 0.12 | [56] | ||
T. glabrata T.G(4ade12)-PMS-P160A | Fumarate | 0.15 | 9.2 | 0.15 | [44] | ||
T. glabrata TGFA091-16 | Fumarate | 0.13 | 33.13 | 0.35 | [58] | ||
T. glabrata T.G-4G-S | Fumarate | 0.09 | 8.51 | 0.12 | [59] | ||
C. glabrata CGhif-6 | Pyruvate | – | 53.1 | 0.74 | [60] | ||
T. glabrata T.G-4G-S(1:1:2)-P(M)-F(H) | Fumarate | – | 21.6 | 0.3 | [59] | ||
C. glabrata C-Δadh-Δald-Δbdh -ScPDC1-NOX | Acetoin |  | 7.33 | 0.11 | [42] | ||
C. glabrata Cgcrz1 ∆ /CgCRZ1 | Pyruvate | – | 1.6-fold | 1.6-fold | [47] | ||
C. glabrata HTUΔ/CgMED15B | Pyruvate |  | 1.61-fold | 1.61-fold | [140] | ||
T. glabrata T. G-KS(H)-S(M)–A-2 S | Fumarate | – | 15.7 | 0.22 | [62] | ||
C. glabrata CmA5 | Acetoin | – | 3.26 | 0.05 | [61] | ||
C. glabrata CGΔura3-SpMPC1 | Pyruvate | – | 2.34-fold | 2.34-fold | [63] | ||
T. glabrata CCTCC M202019 | Pyruvate | 0.44 | 40.2 | 0.56 | [55] | ||
T. glabrata T.G-PMS | L-malate | – | 8.5 | 0.12 | [56] | ||
C. utilis | C. utilis | Glutathione | – | 0.33 | – | [70] | |
C. utilis WSH 02–08 | Glutathione | – | 1.33 | – | [67] | ||
C. utilis | Lipase activity | – | 0.6 (A450) | – | [74] | ||
C. utilis | Biotin | – | 1.8 mg/L | – | [72] | ||
C. utilis TMS411 | Isopropanol | 0.14 | 27.2 | 0.24 | [75] | ||
C. utilis WSH 02–08 | Glutathione | – | 0.74 | – | [68] | ||
C. utilis TMS174 | Ethanol | – | 17.4 | 0.58 | [76] | ||
Cupdc1-null mutant | Lactate | 0.95 | 103.3 | 3.44 | [141] |