Fig. 1

Establishing TAL production in R. toruloides. A Schematic of TAL production using 2-Pyrone Synthase (2-PS) from Gerbera hybrida. 2-PSG was randomly integrated into the genome of R. toruloides IFO0880 (WT) to generate a TAL producing strain. B Growth of WT and TAL producing R. toruloides in YPD, or YPD supplemented with 2 g/L TAL, as tracked in a BioLector Pro microbioreactor. C Optical densities measured at the end of cultivation in the microbioreactor. D Titers of TAL recovered at the end of growth in B during growth in YPD (white bars), or YPD supplemented with 2 g/L TAL (grey bars). No TAL was observed from WT samples grown in YPD, nor was TAL observed in blank YPD. Error bars represent standard deviation of nine biological replicates (R. toruloides cultures) or three blank replicates (Blank)