Table 1 Bacterial strains and plasmids used in this study
From: Metabolic engineering of Clostridium autoethanogenum for ethyl acetate production from CO
Strain | Relevant characteristics | Source or reference |
|---|---|---|
E. coli DH5α | F-endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK +), λ– | NEB (New England Biolabs Inc., Ipswich, MA, United States) |
E. coli sExpress | fhuA2 [lon] ompT gal sulA11 R(mcr-73:miniTn10–TetS)2 [dcm] R(zgb-210:Tn10–TetS) endA1 Δ(mcrC-mrr)114:IS10 carrying the Tra+, Mob+ plasmid R702 [R702-TcR, SmR, SuR, HgR] | [22] |
C. autoethanogenum JA1-1 (DSM 10,061) | Wild type | DSMZ |
C. autoethanogenum ∆pta | ∆pta (CAETHG_3358) | This study |
C. autoethanogenum ∆adhE1a | Knockout of the Ald subunit of adhE1 (CAETHG_3747) | This study |
pMTL83151 | pCB102, ColE1, traJ, CmR/TmR (catP) | [23] |
pMTL83122 | pMTL83121 PThl | [23] |
PPta-AckA-Atf1 | pMTL83151 PPta-AckA-atf1 | This study |
PPta-AckA-Eat1 | pMTL83151 PPta-AckA-eat1 | This study |
PPta-AckA-trEat1 | pMTL83151 PPta-AckA-treat1 | This study |
PThl-Atf1 | pMTL83122 PThl-atf1 | This study |
PThl-Eat1 | pMTL83122 PThl-eat1 | This study |
pRECas1 | pMTL83151, Pfdx*-RbE-cas9 | [24] |
pRECas1-adhE1a | pRECas1, sgRNA and HA targeting adhE1a (CAETHG_3747) | This study |
pSIBR003 | Int3-FnCas12a | [25] |
pMTL-SIBR-Cas | pMTL83151, PFdx- Int3-FnCas12a, PAraE-NTspacer | This study |
pMTL-SIBR-Cas-pta | pMTL-SIBR-Cas with spacer and HA targeting pta (CAETH_3358) | This study |