Table 3 Optimized 5′-proximal coding sequence used in this study
Nucleotide sequence | Free energy of second structure (kcal/mol) | Hairpin position | 5′-Proximal coding sequence |
|---|---|---|---|
ATGATTCAAAAACGAAAGCGGACAGTTTCGTTCAG | − 8.2 | < 13 | Control |
ATGATACAAAAGAGGAAAAGGACGGTTAGTTTTAG | − 3.7 | > 13 | Opt1 |
ATGATACAAAAGCGAAAAAGGACGGTCAGTTTTAG | − 3.7 | None | Opt2 |
ATGATACAAAAACGAAAGAGGACGGTCAGTTTTAG | − 3.8 | > 13 | Opt3 |
ATGATACAGAAACGAAAGCGGACGGTCAGTTTTAG | − 4.1 | None | Opt4 |
ATGATACAAAAACGAAAGAGGACAGTCAGCTTTAG | − 4.2 | > 13 | Opt5 |