Fig. 3
From: Toward more efficient ergothioneine production using the fungal ergothioneine biosynthetic pathway
Plasmid profiles, protein expression and ERG biosynthesis. A Schematic drawing of plasmids expressing tregt1 and/or tregt2 used for transforming E. coil BW25113. B The production of ERG by 48-h whole cell catalysis using the recombinant strains BW-tregt1, BW-tregt2 and BW-tregt1-tregt2. Data in the figure are mean values (n = 3 biological replicates). C Detection of Tregt1 and/or Tregt2 expression in recombinant E. coli by SDS-PAGE. M. Protein marker; 1. BW-pBAD (control); 2. BW-tregt1; 3. BW-tregt2; 4. BW-tregt1-tregt2