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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Co-cultures of Propionibacterium freudenreichii and Bacillus amyloliquefaciens cooperatively upgrade sunflower seed milk to high levels of vitamin B12 and multiple co-benefits

Fig. 1

Screening of food-grade strains of P. freudenreichii for vitamin B12 synthesis. The data comprise the final titer of vitamin B12 in basic MRS medium and in MRS medium, supplemented with 50 µM CoCl2 and 100 µM dimethylbenzimidazole (DMBI) (A). In addition, pH, and growth of selected P. freudenreichii strains in MRS medium (B), and vitamin B12 production and growth of selected P. freudenreichii strains in pasteurized sunflower seed milk medium is shown (C). P. freudenreichii DSM 4902 was used as a positive control. The process was carried out as dual phase cultivation with an initial anaerobic phase (48 h), followed by an aerobic phase (24 h). n = 3

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