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Correction to: Genetic and process engineering strategies for enhanced recombinant N-glycoprotein production in bacteria

The Original Article was published on 14 October 2021

Correction to: Microb Cell Fact (2021) 20:198 https://doi.org/10.1186/s12934-021-01689-x

Following publication of the original article [1], the authors identified an error in Fig. 8. The correct figure (Fig. 8) is given in this correction.

Fig. 8
figure 8

Summary of glycosylation efficiency (%) and glycoprotein titre (mg/L of glycosylated protein) of A scFv13R4, B scFv13R4CM, C RNase A, and D NGRP during production in glyco-competent E. coli K-12 by use of different cultivation conditions to modulate target protein folding. Glycoprotein titres were converted from the yield (mg/g DCW), cell growth, and total protein titre results given in the Figs. 5, 6, 7, and Tables 1, 2, 3, 4 and Additional file 2: Table S6 (“Methods” section). Colour symbols indicate the experiment or figure/table sources for the data (Fig; 1 = Table 1, 2 = Table 2, 3 = Table 3, 4 = Table 4, 5=Additional file 2: Table S6). A similar wt and ΔdsbB cultivation were run in four different batches of experiment, which were (i) wt oxygen transfer experiment (10/50, Fig. 5A–D), (ii) wt against oxidoreductase knockout ΔdsbB (Fig. 6A–D), (iii) wt and ΔdsbB 0 cystine treatment (Fig. 7A–D), (iv) wt against oxidoreductase knockout ΔdsbC (Fig. 7E, F, Additional file 1: Figure S18A, B). A variation of inducer concentration for NGRP expression was used in the experiment of Fig. 6D (200 μM instead of 40 μM PPDA, “Methods” section)

Reference

  1. Pratama F, Linton D, Dixon N. Genetic and process engineering strategies for enhanced recombinant N-glycoprotein production in bacteria. Microb Cell Fact. 2021;20:198. https://doi.org/10.1186/s12934-021-01689-x.

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Correspondence to Neil Dixon.

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Pratama, F., Linton, D. & Dixon, N. Correction to: Genetic and process engineering strategies for enhanced recombinant N-glycoprotein production in bacteria. Microb Cell Fact 20, 210 (2021). https://doi.org/10.1186/s12934-021-01700-5

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  • DOI: https://doi.org/10.1186/s12934-021-01700-5