Table 1 Strains and plasmids used in this work
Names | Characteristics | Source or reference |
|---|---|---|
Strains | ||
BY4742 | S288c, MATα, his3Δ1, leu2Δ0, lys2Δ0, MET15, ura3Δ0 | Thermo Fisher Scientific |
Ypl001 ~ 004,007 ~ 008 | BY4742 derivative, carrying plasmids YPL001 ~ 004, 007 ~ 008 | This study |
BY4742-Kn | BY4742 derivatives, carrying chimeric promoter library with YLP002 as plasmid backbone | This study |
BY4742-Cas9 | BY4742 derivative, carrying plasmid #43,802 | This study |
K0/528/536/540-tHMG1 | BY4742 derivative, HMG1 was truncated in situ and tHMG was expressed using the synthetic minimal promoters K0/528/536/540 | This study |
Plasmids | ||
YPL001 | CDSgfp-TScSPG5 cassette inserted into pRS313 | This study |
YPL002(K0) | UASF-E-C-core11 promoter inserted into YPL001 | This study |
YPL003/004 | Extended/Shortened 5′UTR of K0 | This study |
YPL007/008 | ScTDH3/ScTEF1 promoter inserted into YPL001 | This study |
YPL002-K528 | YPL002 with Kozak variant “GCAATA” | This study |
YPL002-K536 | YPL002 with Kozak variant “CACCAA” | This study |
YPL002-K540 | YPL002 with Kozak variant “ATCGTC” | This study |
p414-TEF1p-Cas9-CYC1t | The Cas9 protein was expressed using a TEF1 promoter and a CYC1 terminator | Addgene plasmid #43802 |
p426-SNR52p-gRNA.CAN1.Y-SUP4t | The gRNA cassette was expressed using an SNR52 promoter and a SUP4 terminator | Addgene plasmid #43803 |