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Table 2 Engineering of protein secretory pathway in yeast

From: Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up

Yeast strain Gene targeted Outcome Reference
S. cerevisiae HSF1 Reduce stress [72]
SCJ1 Involved in protein folding [150]
PDI1 Formation of disulfide bonds [151]
SIL1 Endoplasmic reticulum translocation [63]
JEM1 Chaperone binding
HAC1 Regulation of unfolded protein response [152]
UBI4 Post translation modification [153]
CYM1 Remove protease activity by gene disruption [154]
KEX2 Remove protease activity by gene disruption [155]
VPS10 Enhance protein secretion [156, 157]
MON2 Deletion prevents vesicle formation and enhance secretion [158]
SSO Helps in transportation from golgi to plasma membrane [159]
ALG3, ALG11 Formation of N glycans similar to humans [92]
OCH1 Elongation during glycosylation [160]
MNN4 Mannosylphosphorylation [85]
S. pombe PPP20 Disruption of aminopeptidase [79, 161]
ATG4 Disruption of cysteine protease
FMA2 Disruption of methionine aminopeptidase
OMA1 Disruption of metalloendopeptidase
FMA2 Disruption of methionine aminopeptidase
VPS10 Enhance protein secretion
OMH1 Modifying O linked glycans [162]
GMS1 UDP galactose transportation [87]
P. pastoris SSA1 Protein folding [67, 68]
SSA4
SSE1
SEC63 Transportation of proteins [155]
CUP5 [67]
BFR2
KIN2
OCH1 Decrease addition of mannose [89]
ALG3 Proper glycosylation
YPS1 Diminish protease activity [163]
K. lactis PDI1 Disulfide bond formation [65, 164]
ERO1 Folding of protein [65]
OCH1 Decrease addition of mannose [88]
MNN1 Decrease addition of mannose [12]
Y. lipolitica OCH1 Decrease addition of mannose [88]
H. polymorpha CNE1 Protein folding [165]
ALG3 Proper glycosylation [86]
OCH1 Decrease addition of mannose [86]