Table 2 Engineering of protein secretory pathway in yeast

S. cerevisiae

HSF1

Reduce stress

[72]

SCJ1

Involved in protein folding

[150]

PDI1

Formation of disulfide bonds

[151]

SIL1

Endoplasmic reticulum translocation

[63]

JEM1

Chaperone binding

HAC1

Regulation of unfolded protein response

[152]

UBI4

Post translation modification

[153]

CYM1

Remove protease activity by gene disruption

[154]

KEX2

Remove protease activity by gene disruption

[155]

VPS10

Enhance protein secretion

[156, 157]

MON2

Deletion prevents vesicle formation and enhance secretion

[158]

SSO

Helps in transportation from golgi to plasma membrane

[159]

ALG3, ALG11

Formation of N glycans similar to humans

[92]

OCH1

Elongation during glycosylation

[160]

MNN4

Mannosylphosphorylation

[85]

S. pombe

PPP20

Disruption of aminopeptidase

[79, 161]

ATG4

Disruption of cysteine protease

FMA2

Disruption of methionine aminopeptidase

OMA1

Disruption of metalloendopeptidase

FMA2

Disruption of methionine aminopeptidase

VPS10

Enhance protein secretion

OMH1

Modifying O linked glycans

[162]

GMS1

UDP galactose transportation

[87]

P. pastoris

SSA1

Protein folding

[67, 68]

SSA4

SSE1

SEC63

Transportation of proteins

[155]

CUP5

[67]

BFR2

KIN2

OCH1

Decrease addition of mannose

[89]

ALG3

Proper glycosylation

YPS1

Diminish protease activity

[163]

K. lactis

PDI1

Disulfide bond formation

[65, 164]

ERO1

Folding of protein

[65]

OCH1

Decrease addition of mannose

[88]

MNN1

Decrease addition of mannose

[12]

Y. lipolitica

OCH1

Decrease addition of mannose

[88]

H. polymorpha

CNE1

Protein folding

[165]

ALG3

Proper glycosylation

[86]

OCH1

Decrease addition of mannose

[86]