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Table 4 List of PCR primers used in the study

From: Protective effects of a food-grade recombinant Lactobacillus plantarum with surface displayed AMA1 and EtMIC2 proteins of Eimeria tenella in broiler chickens

Primer

Primer sequences (5′–3′) for PCR sequence

P-EGFP F

GAAAATTAGTTACCAGAAAGTGTCTAGAATGGTGAGCAAGGG (XbaI)

P-EGFP R

AGCAACACGTGCTGTAATTTGAAGCTTTTACTTGTACAGCTCGTC (HindIII)

S-EGFP F

GGCACGATTGCGGCGGTCGACATGGTGAGCAAGGGCGAGG (SalI)

S-EGFP R

GCATGGTACCGAATTCCTCGAGCTTGTACAGCTCGTCCATG (XhoI)

asd F

GCACCGCTATGCGTGCGGGATCCTCTTCCCTAAATTTAAAT (BamHI)

asd R

GTCCACAACATCAGGTAGTG

P23 F

TGTCACTACCTGATGTTGTGGACGAAAAGCCCTGACAAC

alr R

CAAATTTAAAAAAGCGACTCATAGAATTAATCTATATAAACTCTCG

pF

TTCTATGAGTCGCTTTTTTAAATTTG

pR

GGATCCCGCACGCATAGCGGTGC

SD-P′ MF

GCAAACTTTAGATAATAAAAaCTTAGGAAACAGACCATGGGCAAG AAAGAAT

SD-P′ M R

GCAACACGTGCTGTAATTTGAAGCTTTTAATGGTGATGGTGATGATG

SD-EGFPS F

TCATCACCATCACCATTAAAAGCTTAGGAAACAGACCATGAAGAA AAATTTAAG

SD-EGFPS R

CACGTGCTGTAATTTGAAGCTTTTATTATCTAAAG TTTGC

  1. aLetters in italics indicate the introduction of restriction sites
  2. bUnderlined text indicates extensions of about 15–25 bp that were complementary to the ends of the digested vector or anchoring gene fragments for seamless cloning or overlap extension (SOE)-PCR
  3. cBoldface letters indicate SD
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Microbial Cell Factories

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