Protective effects of a food-grade recombinant Lactobacillus plantarum with surface displayed AMA1 and EtMIC2 proteins of Eimeria tenella in broiler chickens

Table 2 Protective effects in each group

Groups	Survival rate (%)	Body weight gain (g)	Relative body weight gain rate (%)	Lesion scores in cecum	Oocyst output (× 10⁵)	Oocyst decrease ratio (%)
PBS	100	337.40 ± 11.71^A	100	0	0	100
PBS-challenge	100	210.50 ± 16.16^B	62.39	3.83 ± 0.41^A	9.50 ± 3.03^A	0
Lp/pLQa-pgsA′	100	234.20 ± 22.48^C	69.41	3.33 ± 0.82^A	7.41 ± 2.16^A	22.00
Lp-12	100	313.71 ± 6.60^D	92.98	2.00 ± 0.63^B	3.56 ± 1.30^B	62.53

Different character means different statistical significance. Statistically significant among numbers with different characters (P > 0.05). No statistically significant among numbers with the same characters (P < 0.05). PBS group and PBS-challenge control group were treated with 200 μL PBS, p.o. The experimental (Lp-12) group was treated with Lp-12 (2 × 10⁹ CFU/200 μL), p.o. Animals in the vector control group were treated with Lp/pLQa-pgsA′ (2 × 10⁹ CFU/200 μL) at 4–6 days of age. A booster vaccination of Lp/pLQa-pgsA′ (2 × 10⁹ CFU/200 μL) was administered when animals were 18–20 days of age. Ten days after the immunization (30 days of age), chickens (other than those in the PBS control group) were challenged with 5 × 10⁴E. tenella sporulated oocysts

ISSN: 1475-2859