Fig. 7
From: Identification of a Yarrowia lipolytica acetamidase and its use as a yeast genetic marker
Plasmid curing through fluoroacetamide counterselection. a Acetamide-positive pNC1344-YlAMD1 transformants were plated on fluoroacetamide media for counterselection of the acetamidase plasmid. b Parent strains Y. lipolytica NS995 (“Yl”) and S. cerevisiae S288C (“Sc”), acetamide-positive pNC1344-YlAMD1 transformants and fluoroacetamide-resistant isolates derived from these transformants were patched on defined media containing either 5 g/L ammonium sulfate or 0.56 g/L acetamide as the sole nitrogen source and incubated at 30 °C for 2 days. c The strains in B were probed for the presence of the plasmid using primers specific for the YlAMD1 gene