Fig. 4
From: Efficient production of myo-inositol in Escherichia coli through metabolic engineering
Effects of regulating zwf and deleting pgm on inositol production. a SDS-PAGE showing the expression of IPS (approximately 60 kDa) and IMP (approximately 30 kDa). b Production of inositol in different host strains. The recombinant strains were induced and harvested, then suspended in a bioconversion mixture containing 50 mM glucose. The bioconversions were performed at 37 °C and 220 rpm for 10 h