Fig. 4

Sulfotransferase in notonesomycin B biosynthesis a Schematic of O-sulfation of notonesomycin A by a PAPS-dependent sulfotransferase during notonesomycin B biosynthesis; b HPLC-coupled UV and mass analyses of extracts from wild type and nbc48 KO mutant. Shown is the extracted ion chromatograms (EICs) of extracts from WT and nbc48 KO strain. Arrow indicate accumulated notonesomycin A (m/z 1454.6985 [M+H]⁺) and notonesomycin B (m/z 1374.7414 [M+H]⁺); c Relative abundances of notonesomycins A and B in WT and nbc48 KO mutant in NotA culture conditions (EIC counts 10⁷)