Fig. 3
From: Identification and engineering of 32 membered antifungal macrolactone notonesomycins
Relative gene expression associated with notonesomycin biosynthesis of nbc14 overexpression (OE) mutant in a SV2 and c CA10LB media, was determined by RT-qPCR. Log2 fold changes were shown relative to wild type control. Error bars refer to standard deviation for three independent experiments. Relative abundances of notonesomycins A and B of Streptomyces sp. A793 WT and nbc14 OE mutant in b SV2 (EIC counts 107) and d CA10LB media (EIC counts 103). The increase in notonesomycin production in both media was statistically significant with P < 0.001. Counts were obtained from MS-extracted ion chromatograph (EIC) of notonesomycin A (m/z 1454.6985 [M+H]+) and notonesomycin B (m/z 1374.7414 [M+H]+)