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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Relative contributions of non-essential Sec pathway components and cell envelope-associated proteases to high-level enzyme secretion by Bacillus subtilis

Fig. 2

Secretion of AmyE, AmyL or BPN’ by strains lacking individual non-essential secretion machinery components. AmyE-, AmyL- or BPN’-producing strains lacking the dnaK, secDF, secG, sipS, sipT, sipU, sipV or sipW genes, as well as the respective wild-type (wt) control, were grown for 16 h in MBU medium at 37 °C. Next, cells and growth media were separated by centrifugation and proteins in the growth medium fractions were analyzed by LDS-PAGE and SimplyBlue SafeStaining as described for Fig. 1

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Microbial Cell Factories

ISSN: 1475-2859

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