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Table 1 Volumetric and specific productivity as well as product yield and specific growth rate of the fed-batch cultivation of HyHEL-Fab#34 overexpressing SBH1, deleted in ypt7 and vam6pep4 and combinations thereof

From: Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris

  Mean Qp [µg L−1 h−1] Mean qp [µg g−1 h−1] YX/S [g g−1] Mean µ [h−1]
Fab#34 0.25 ± 0.03 3.35 ± 0.28 0.443 ± 0.038 0.022 ± 0.001
Δvam6Δpep4 0.42 5.83 0.471 0.021
Δvam6Δpep4 + SBH1OE 0.50 ± 0.06 6.36 ± 0.94 0.462 ± 0.012 0.0255 ± 0.0025
Δypt7 0.40 ± 0.03 5.85 ± 0.43 0.430 ± 0.043 0.023 ± 0.001
SBH1OE 0.42 4.81 0.367 0.021
Δypt7 + SBH1OE 0.62 ± 0.00 9.36 ± 0.32 0.468 ± 0.008 0.0225 ± 0.0015
  1. For Fab#34 mean ± SD from three individual fed batch cultivations are shown, for Δypt7, Δvam6Δpep4 + SBH1OE and Δypt7 + SBH1OE two individual fed batch cultivations were performed