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Table 1 Volumetric and specific productivity as well as product yield and specific growth rate of the fed-batch cultivation of HyHEL-Fab#34 overexpressing SBH1, deleted in ypt7 and vam6pep4 and combinations thereof

From: Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris

 

Mean Qp [µg L−1 h−1]

Mean qp [µg g−1 h−1]

YX/S [g g−1]

Mean µ [h−1]

Fab#34

0.25 ± 0.03

3.35 ± 0.28

0.443 ± 0.038

0.022 ± 0.001

Δvam6Δpep4

0.42

5.83

0.471

0.021

Δvam6Δpep4 + SBH1OE

0.50 ± 0.06

6.36 ± 0.94

0.462 ± 0.012

0.0255 ± 0.0025

Δypt7

0.40 ± 0.03

5.85 ± 0.43

0.430 ± 0.043

0.023 ± 0.001

SBH1OE

0.42

4.81

0.367

0.021

Δypt7 + SBH1OE

0.62 ± 0.00

9.36 ± 0.32

0.468 ± 0.008

0.0225 ± 0.0015

  1. For Fab#34 mean ± SD from three individual fed batch cultivations are shown, for Δypt7, Δvam6Δpep4 + SBH1OE and Δypt7 + SBH1OE two individual fed batch cultivations were performed
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Microbial Cell Factories

ISSN: 1475-2859

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