Fig. 2

Vacuolar membrane staining of a Fab#34, ∆vam6, ∆vps41, ∆ypt7 and b CES#18, ∆vam6, ∆vps41, ∆ypt7. The vacuolar membrane was stained with FM4-64 and the cells were viewed in an epifluorescence microscope. The fluorescence and the DIC images are shown. Per strain at least 300–500 cells were analysed in at least 10 microscopic images, of which 100% show the described phenotype. Two representative full size microscopic images per strain are shown in Additional file 2: Figure S2. Bar, 3 µm