Table 2 Strains involved in this study
From: Heterologous production of levopimaric acid in Saccharomyces cerevisiae
Strains | Description | Source |
|---|---|---|
W303-1a | MATa; leu2-3112; trp1-1; can1-100; ura3-1; ade2-1; his3-11,15 | Our lab |
WTI | W303-1a; δ:: PPGK1- tHMG1-TPGK1, TTDH3-IDI1-TADH1 | This study |
WTI-BE | W303-1a; δ:: PPGK1- tHMG1-TPGK1, TTDH3-IDI1-TADH1, PPGK1-BTS1-GGGS-ERG20- TCYC1 | This study |
W1 | W303-1a; δ:: PPGK1- tHMG1-TPGK1, TTDH3-IDI1-TADH1, PPGK1-BTS1-GGGS-ERG20- TCYC1; ura3:: PTEF1-LPS-TADH2 | This study |
W2 | W303-1a; δ:: PPGK1- tHMG1-TPGK1, TTDH3-IDI1-TADH1, PPGK1-BTS1-GGGS-ERG20- TCYC1; ura3:: PTEF1-LPSMM-TADH2 | This study |
W3 | W303-1a; δ:: PPGK1- tHMG1-TPGK1, TTDH3-IDI1-TADH1, PPGK1-BTS1-GGGS-ERG20- TCYC1; ura3:: PTEF1-T40LPS-TADH2 | This study |
W4 | W303-1a; δ:: PPGK1- tHMG1-TPGK1, TTDH3-IDI1-TADH1, PPGK1-BTS1-GGGS-ERG20- TCYC1; ura3:: PTEF1-T40LPSMM-TADH2 | This study |
W5 | W303-1a; δ:: PPGK1- tHMG1-TPGK1, TTDH3-IDI1-TADH1, PPGK1-BTS1-GGGS-ERG20- TCYC1; ura3:: PTEF1-T60LPS-TADH2 | This study |
W6 | W303-1a; δ:: PPGK1- tHMG1-TPGK1, TTDH3-IDI1-TADH1, PPGK1-BTS1-GGGS-ERG20- TCYC1; ura3:: PTEF1-T60LPSMM-TADH2 | This study |
W7 | W303-1a; δ:: PPGK1- tHMG1-TPGK1, TTDH3-IDI1-TADH1, PPGK1-BTS1-GGGS-ERG20- TCYC1; ura3:: PTEF1-T79LPS-TADH2 | This study |
W8 | W303-1a; δ:: PPGK1- tHMG1-TPGK1, TTDH3-IDI1-TADH1, PPGK1-BTS1-GGGS-ERG20- TCYC1; ura3:: PTEF1-T79LPSMM-TADH2 | This study |
WTE | W303-1a; ade2:: PPGK1- tHMG1-TPGK1, TTDH3-ERG20-TERG20 | This study |
W9 | W303-1a; ade2:: PPGK1- tHMG1-TPGK1, TTDH3-ERG20-TERG20; PERG9::PMET3-ERG9 | This study |
W10 | W303-1a; δ:: PPGK1- tHMG1-TPGK1, TTDH3-IDI1-TADH1, ura3:: PTEF1-T79LPSMM-TADH1, PTEF1-BTS1-TADH2 | This study |
W11 | W303-1a; ade2:: PPGK1- tHMG1-TPGK1, TTDH3-ERG20-TERG20, ura3:: PTEF1-T79LPSMM-TADH1, PTEF1-BTS1-TADH2 | This study |
W12 | W303-1a; ade2:: PPGK1- tHMG1-TPGK1, TTDH3-ERG20-TERG20; PERG9::PMET3-ERG9; ura3:: PTEF1-T79LPSMM-TADH1, PTEF1-BTS1-TADH2 | This study |
WM | W303-1a; ade2:: PPGK1- tHMG1-TPGK1, TTDH3-ERG20-TERG20; PERG9::PMET3-ERG9; rDNA:: PTEF1-T79LPSMM-TADH1, PTEF1-BTS1-TADH2 | This study |
WM1 | W303-1a; ade2:: PPGK1- tHMG1-TPGK1, TTDH3-ERG20-TERG20; PERG9::PMET3-ERG9; rDNA:: PTEF1-T79LPSMM-TADH1, PTEF1-BTS1-TADH2; his3:: PPGK1-CYP720B1-TADH1, PTDH3-AtCPR1-TTDH2 | This study |
WM2 | W303-1a; ade2:: PPGK1- tHMG1-TPGK1, TTDH3-ERG20-TERG20; PERG9::PMET3-ERG9; rDNA:: PTEF1-T79LPSMM-TADH1, PTEF1-BTS1-TADH2; his3:: PPGK1-CYP720B1-TADH1, PTDH3-AtCPR2-TTDH2 | This study |
WM3 | W303-1a; ade2:: PPGK1- tHMG1-TPGK1, TTDH3-ERG20-TERG20; PERG9::PMET3-ERG9; rDNA:: PTEF1-T79LPSMM-TADH1, PTEF1-BTS1-TADH2; his3:: PPGK1-CYP720B1-TADH1, PTDH3-TcCPR-TTDH2 | This study |
WMM | W303-1a; ade2:: PPGK1- tHMG1-TPGK1, TTDH3-ERG20-TERG20; PERG9::PMET3-ERG9; rDNA:: PTEF1-T79LPSMM-TADH1, PTEF1-BTS1-TADH2; δ:: PPGK1-CYP720B1-TADH1, PTDH3-TcCPR-TTDH2 | This study |