Fig. 3
From: Heterologous production of levopimaric acid in Saccharomyces cerevisiae
LPS modification improves LP production. a Homology modeling comparison between LPS (green) and T79LPS (blue); conserved DDXXD motif is marked in orange and the N-terminal plastid transit peptide is shown in red; circles in dotted lines show the differences between them. b Homology modeling of LPS; N-terminal 79 amino acid is shown in red; Met593 and Tyr700, located in the α domain binding pocket, are shown in orange; the conserved DDXXD motif is also in orange. c Homology modeling of T79LPSMM; Ile593 and Phe700, located in the α domain binding pocket, are shown in pink; the conserved DDXXD motif is also in pink. d LP and GGOH production by different strains with various LPS modifications: W1 (LPS), W2 (LPSM593I/Y700F), W3 (T40LPS), W4 (T40LPSM593I/Y700F), W5 (T60LPS), W6 (T60 LPSM593I/Y700F), W7 (T79LPS), and W8 (T79 LPSM593I/Y700F); Truncation is abbreviated to “T” and the number refers to the amino acid removed from the N-terminal. Error bars represent the standard deviation from three independent experiments