Fig. 2

Single repression of the pyc gene and gltA gene by the two-plasmid CRISPRi system in C. glutamicum wild-type (WT). The wild-type and Pyc-targeting recombinant cells were cultivated with either 111 mM glucose or 178 mM sodium lactate as sole carbon (a; left panel). The wild-type and CS-targeting recombinant cells were cultivated with either 111 mM glucose or 150 mM sodium acetate as the sole carbon source (b; right panel). RT-PCR and quantitative RT-PCR were performed to investigate the levels of mRNA expression of the pyc gene, the gltA gene, and the sigA gene as a control for the wild-type and the recombinants with sgRNAs grown with 111 mM glucose. Data represents mean values of at least three cultivations and error bars represent standard deviations