Fig. 5

Fed-batch feeding strategy results in significant resorcinol metabolism over a prolonged period. Growth (closed circles, solid lines) are on the left axis and substrate utilization (open circles, dashed lines) are on the right axis. Initial TOLN media contained 3 g/L resorcinol. After 36 h, cells were fed with 2 g/L resorcinol in 24 h intervals while keeping culture volume constant. Cultures show full resorcinol consumption after each feeding; however, cell growth plateaus, potentially indicating high lipid accumulation in the late phase of culturing. The data are the mean and error bars are standard deviation of biological replicates (n = 5). Lines are used only for visual aid