Fig. 2
From: Generation of a platform strain for ionic liquid tolerance using adaptive laboratory evolution
Performance of evolved clones in batch culture under M9 minimal media conditions with various [C2C1Im][OAc] IL loadings. Shown is the final optical density (OD600nm) of the selected best performing clones (Table 3) under different concentrations of [C2C1Im][OAc]: a first, a screen with 250 mM and all best performing clones, b second, a follow up screen with 300, 500 and 700 mM utilizing the highest final density clones from the 250 mM screen. Surprisingly, a number of clones that were originally evolved for [C4C1Im]Cl tolerance showed high cross-tolerance to elevated [C2C1Im][OAc] concentrations. At the highest concentration (700 mM), only the MG1655 derived strains showed measurable growth