Fig. 2
Time course studies to determine biomass, total lipid yield and lipid content of MNNG and MNNG + cerulenin treated mutants. Mutants a YlB6, b YlC7 and c YlE1were grown on 100 g L−1 WCO as mentioned in “Methods”. All values are represented as mean ± SD, determined after three independent experiments. Biomass (g L−1)—black down pointing triangle, Total lipid yield (g L−1)—black circle, Lipid content (%)—white circle. Inset: In each graph light microscopy (left panel) and Nile red fluorescence microscopy (right panel) images of the respective Y. lipolytica mutants under 100 × oil immersion objective. Bar indicates 4 μm