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Table 1 Combinatorial effect of various process parameters on the solubility and activity of rHSA

From: Revisiting Escherichia coli as microbial factory for enhanced production of human serum albumin

Process condition Intracellular environment Post induction temperature (°C) Induction type Osmolyte assistance Solubility (% of total rHSA expressed) Fold increase in activity
A Reducing 37 IPTG No 3 1
B Reducing 25 IPTG No 4 ~ 6
C Reducing 18 IPTG No 6 ~ 4
D Reducing 37 Autoinduction No 14 ~ 10
E Reducing 25 Autoinduction No 12 ~ 9
F Reducing 18 Autoinduction No 17 ~ 16
G Oxidizing 37 IPTG No 4.2 ~ 1
H Oxidizing 25 IPTG No 24.8 ~ 32
I Oxidizing 18 IPTG No 22 ~ 32
J Oxidizing 37 Autoinduction No 9.4 ~ 5
K Oxidizing 25 Autoinduction No 35.7 ~ 42
L Oxidizing 18 Autoinduction No 52.5 ~ 53
M Oxidizing 18 Autoinduction Yes 61 ~ 100