Table 1 Combinatorial effect of various process parameters on the solubility and activity of rHSA
From: Revisiting Escherichia coli as microbial factory for enhanced production of human serum albumin
Process condition | Intracellular environment | Post induction temperature (°C) | Induction type | Osmolyte assistance | Solubility (% of total rHSA expressed) | Fold increase in activity |
|---|---|---|---|---|---|---|
A | Reducing | 37 | IPTG | No | 3 | 1 |
B | Reducing | 25 | IPTG | No | 4 | ~Â 6 |
C | Reducing | 18 | IPTG | No | 6 | ~Â 4 |
D | Reducing | 37 | Autoinduction | No | 14 | ~Â 10 |
E | Reducing | 25 | Autoinduction | No | 12 | ~Â 9 |
F | Reducing | 18 | Autoinduction | No | 17 | ~Â 16 |
G | Oxidizing | 37 | IPTG | No | 4.2 | ~Â 1 |
H | Oxidizing | 25 | IPTG | No | 24.8 | ~Â 32 |
I | Oxidizing | 18 | IPTG | No | 22 | ~Â 32 |
J | Oxidizing | 37 | Autoinduction | No | 9.4 | ~Â 5 |
K | Oxidizing | 25 | Autoinduction | No | 35.7 | ~Â 42 |
L | Oxidizing | 18 | Autoinduction | No | 52.5 | ~Â 53 |
M | Oxidizing | 18 | Autoinduction | Yes | 61 | ~Â 100 |