Fig. 6

Time course of citramalate production by E. coli MEC568/pZE12-cimA (∆gltA ∆leuC ∆ackA-pta ∆poxB) in fed-batch culture. The defined medium initially contained 30 g/L glucose, 5 g/L l-glutamate and 1.0 g/L l-leucine. When the glucose concentration decreased below 5 g/L, 20.0 g glucose, 5.0 g l-glutamate and 1.0 g l-leucine dissolved together in 35 mL DI water were added four times