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Table 3 Effect of evolution-induced dgcQ C1081A mutation on phototrophic growth and proton pumping

From: An evolutionary optimization of a rhodopsin-based phototrophic metabolism in Escherichia coli

Strain Genotype Growth in dark (A) Growth in light (B) Phototrophic growth Δ[B − A]a Proton pumping (ΔH+ × 10−7/min OD)
HK774 Ancestral E. coli amyA::KmR, dgcQ WT 0.98 ± 0.02 1.08 ± 0.04 0.10 0.475 ± 0.020
HK775 Ancestral E. coli amyA::KmR, dgcQ C1082A 1.13 ± 0.04 1.64 ± 0.03 0.51 0.903 ± 0.107
  1. aPhototrophic growth was estimated from the difference of the growth in light conditions (B) and the growth in dark conditions (A). Data of the growths represent the mean of optical densities ± SD at 600 nm from at least three biological repeats