An evolutionary optimization of a rhodopsin-based phototrophic metabolism in Escherichia coli

Table 3 Effect of evolution-induced dgcQ ^C1081A mutation on phototrophic growth and proton pumping

Strain	Genotype	Growth in dark (A)	Growth in light (B)	Phototrophic growth Δ[B − A]^a	Proton pumping (ΔH⁺ × 10⁻⁷/min OD)
HK774	Ancestral E. coli amyA::KmR, dgcQ ^WT	0.98 ± 0.02	1.08 ± 0.04	0.10	0.475 ± 0.020
HK775	Ancestral E. coli amyA::KmR, dgcQ ^C1082A	1.13 ± 0.04	1.64 ± 0.03	0.51	0.903 ± 0.107

^aPhototrophic growth was estimated from the difference of the growth in light conditions (B) and the growth in dark conditions (A). Data of the growths represent the mean of optical densities ± SD at 600 nm from at least three biological repeats

ISSN: 1475-2859