Skip to main content

Table 1 Heterologous production of sesquiterpenes in S. cerevisiae BJ5464 expressing GME3634, GME3638, and GME9210

From: Heterologous expression of cytotoxic sesquiterpenoids from the medicinal mushroom Lignosus rhinocerotis in yeast

RT (min) SI (%) Peak area Formula Name Structure
GME3634
 10.315 65 400,382 (C15H24) Unknown sesquiterpene  
 10.790 74 282,208 (C15H24) Unknown sesquiterpene  
 10.855 68 187,589 (C15H24) Unknown sesquiterpene  
 11.370 91 3,367,644 C15H26O Germacrene D-4-ol
 11.820 75 66,158 (C15H26O) Unknown sesquiterpene  
 12.035 89 525,206 C15H26O τ-Muurolol
 12.080 74 84,666 (C15H26O) Unknown sesquiterpene  
 12.175 95 4,408,840 C15H26O α-Cadinol
GME3638
 9.510 92 501,063 C15H24 α-Copaene
 9.590 88 506,236 C15H24 β-Elemene
 9.655 94 4,396,543 C15H24 β-Elemene
 9.915 76 197,793 (C15H24) Unknown sesquiterpene  
 10.200 86 764,201 C15H24 Isoledene
 10.315 55 707,826 (C15H24) Unknown sesquiterpene  
 10.430 89 1,820,157 C15H24 Germacrene D
 10.480 94 1,041,092 C15H24 Germacrene D
 10.590 87 1,068,119 C15H24 β-Cubebene
 10.645 95 2,408,088 C15H24 α-Muurolene
 10.855 83 1,246,428 C15H24 δ-Cadinene
 10.940 68 603,336 (C15H24) Unknown sesquiterpene  
 11.455 69 254,072 (C15H24) Unknown sesquiterpene  
 10.795 82 3,768,107 C15H26O Selina-6-en-4-ol
 11.375 94 8,944,970 C15H26O Germacrene D-4-ol
 11.510 75 963,361 (C15H26O) Unknown sesquiterpene  
 11.830 86 805,039 C15H26O Germacrene D-4-ol
 12.100 95 41,559,921 C15H26O Torreyol
 12.180 93 1,713,657 C15H26O α-Cadinol
GME9210
 9.535 88 734,814 C15H24 1,3,4,5,6,7-Hexahydro-2,5,5-trimethyl-2H-2,4a-ethanonaphthalene
 11.375 74 512,708 (C15H26O) Unknown sesquiterpene  
  1. Possible candidate with the highest SI is shown for compound identification. Sesquiterpenes with SI lower than 80 but with predicted molecular formula typical of sesquiterpenes are listed as unknown sesquiterpene
  2. RT retention time, SI similarity index