Heterologous expression of cytotoxic sesquiterpenoids from the medicinal mushroom Lignosus rhinocerotis in yeast

Table 1 Heterologous production of sesquiterpenes in S. cerevisiae BJ5464 expressing GME3634, GME3638, and GME9210

RT (min)	SI (%)	Peak area	Formula	Name
GME3634
10.315	65	400,382	(C₁₅H₂₄)	Unknown sesquiterpene
10.790	74	282,208	(C₁₅H₂₄)	Unknown sesquiterpene
10.855	68	187,589	(C₁₅H₂₄)	Unknown sesquiterpene
11.370	91	3,367,644	C₁₅H₂₆O	Germacrene D-4-ol
11.820	75	66,158	(C₁₅H₂₆O)	Unknown sesquiterpene
12.035	89	525,206	C₁₅H₂₆O	τ-Muurolol
12.080	74	84,666	(C₁₅H₂₆O)	Unknown sesquiterpene
12.175	95	4,408,840	C₁₅H₂₆O	α-Cadinol
GME3638
9.510	92	501,063	C₁₅H₂₄	α-Copaene
9.590	88	506,236	C₁₅H₂₄	β-Elemene
9.655	94	4,396,543	C₁₅H₂₄	β-Elemene
9.915	76	197,793	(C₁₅H₂₄)	Unknown sesquiterpene
10.200	86	764,201	C₁₅H₂₄	Isoledene
10.315	55	707,826	(C₁₅H₂₄)	Unknown sesquiterpene
10.430	89	1,820,157	C₁₅H₂₄	Germacrene D
10.480	94	1,041,092	C₁₅H₂₄	Germacrene D
10.590	87	1,068,119	C₁₅H₂₄	β-Cubebene
10.645	95	2,408,088	C₁₅H₂₄	α-Muurolene
10.855	83	1,246,428	C₁₅H₂₄	δ-Cadinene
10.940	68	603,336	(C₁₅H₂₄)	Unknown sesquiterpene
11.455	69	254,072	(C₁₅H₂₄)	Unknown sesquiterpene
10.795	82	3,768,107	C₁₅H₂₆O	Selina-6-en-4-ol
11.375	94	8,944,970	C₁₅H₂₆O	Germacrene D-4-ol
11.510	75	963,361	(C₁₅H₂₆O)	Unknown sesquiterpene
11.830	86	805,039	C₁₅H₂₆O	Germacrene D-4-ol
12.100	95	41,559,921	C₁₅H₂₆O	Torreyol
12.180	93	1,713,657	C₁₅H₂₆O	α-Cadinol
GME9210
9.535	88	734,814	C₁₅H₂₄	1,3,4,5,6,7-Hexahydro-2,5,5-trimethyl-2H-2,4a-ethanonaphthalene
11.375	74	512,708	(C₁₅H₂₆O)	Unknown sesquiterpene

Possible candidate with the highest SI is shown for compound identification. Sesquiterpenes with SI lower than 80 but with predicted molecular formula typical of sesquiterpenes are listed as unknown sesquiterpene
RT retention time, SI similarity index

ISSN: 1475-2859