Fig. 4
From: Chromosome engineering of Escherichia coli for constitutive production of salvianic acid A
Construction of plasmid-free and inducer-free strain BKD13 for constitutive production of SAA. a Integration of module 3 into the locus between nupG and speC. Lane 1 nupG-speC fragment in strain BAK10; lane 2 nupG-M3-Chl-speC fragment in strain BAK11-1; lane 3 nupG-M3-speC fragment in strain BAK11; primers nupG F and speC R were used for all PCR verification. M: DNA marker. b The OD600 and titer of BKD13 with different initial glucose concentration. c Fed‐batch fermentation of BKD13 with glucose in shake flasks