Fig. 2
From: A new-generation of Bacillus subtilis cell factory for further elevated scyllo-inositol production
Organization of simultaneous overexpression of IolG-IolW (I), IolG-IolW- IolT (II), and PntA-PntB (III). Overexpression of IolG and IolW was constructed in a previous study [15] (I). For simultaneous overexpression of IolG, IolW, and IolT, PCR fragments covering regions A + B + C were ligated by recombinant PCR and integrated into the amyE locus by a double crossover event (II). For overexpression of PntA and PntB, PCR fragments covering regions D + E + F + G + H were ligated and integrated into the vpr locus by a double crossover event (III)