Fig. 1
From: Type IIs restriction based combinatory modulation technique for metabolic pathway optimization
Illustration of β-carotene synthesis pathway in E. coli DXS37-IDI46 (pACYC184-AL-mva). This route involved β-carotene synthesis module, MEP module and MVA module. MVA Genes modulated with TRCM were underlined. G-3-P glyceraldehyde-3-phosphate, DXP 1-deoxy-d-xylulose-5-phosphate, MEP 2C-methyl-d-erythritol-4-phosphate, CDP-ME 4-diphospho-cytidyl-2C-methyl-d-erythritol, CDP-MEP 4-diphosphocytidyl-2C-methyl-d-erythritol-2-phosphate, MEC 2C-methyl-d-erythritol-2,4-cyclodiphosphate, HMBPP 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate, HMG-CoA 3-hydroxy-3-methyl-glutaryl-coenzyme A, MVA mevalonate, MVA-5-P mevalonate-5-phosphate, MVA-5-PP mevalonate-5-diphosphate, IPP isopentenyl diphosphate, DMAPP dimethylallyl diphosphate, GPP geranyl diphosphate, FPP farnesyl diphosphate, GGPP geranylgeranyl diphosphate