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Fig. 5 | Microbial Cell Factories

Fig. 5

From: SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae

Fig. 5

Implementing and exploiting the regulatory state of SWITCH. a Localization of the 15 gRNAs tested for GAL2 promoter activation. b Screening of the efficiency of the 15 gRNAs to guide Cas9 to the GAL2 promoter using TAPE. c Evaluation of ADE2 expression in colony patches on both SC-Ade-Leu and SC-Leu solid media. d Determination of ADE2 transcript levels relative to ACT1 by qRT-PCR in selected strains as indicated. Stars above columns indicate strains with an ADE2 expression level, which is significantly different (p values <0.005) from the corresponding level obtained from a control strain harboring pRS415

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Microbial Cell Factories

ISSN: 1475-2859

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