Fig. 4

By SWITCH, the cas9 gene can be efficiently replaced by dcas9 and dcas9_VP64 genes. a dcas9 and dcas9_VP64 fragments efficiently rescue DNA DSBs formed by Cas9-gRNA_16 in the cas9 gene. b Confirmation of SWITCH gene replacements by PCR of 12 randomly selected clones co-transformed with gRNA_16 and (top) the dcas9 rescue fragment and (bottom) the dcas9_VP64 fragment. Diagnostic PCR fragments for dcas9 and dcas9_VP64 are 821 and 977 bp, respectively