Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: the role of the transcriptional co-repressor complex Cyc8–Tup1 involved in catabolic repression

Table 2 Growth parameters from S. cerevisiae strains cultured with sucrose with or without 2-deoxy-d-glucose

Strain	µ_suc (h⁻¹)	µ_suc-2DG (h⁻¹)	µ_suc/µ _suc-2DG	OD_{max suc}	OD_{max suc-2DG}	OD_{max suc}/OD_{max suc-2DG}
S288c	0.165 ± 0.002	0.074 ± 0.002**	2.23	7.2 ± 0.2	2.4 ± 0.1**	3.0
Sccyc8 ⁻ + YEpNP-CYC8	0.105 ± 0.003	0.082 ± 0.003**	1.28	5.2 ± 0.2	3.73 ± 0.03**	1.39
Sc cyc8⁻ + YEpNP	0.068 ± 0.001	0.072 ± 0.001	0.94	2.7 ± 0.2	2.5 ± 0.3	1.08
Sctup1 ⁻ + YEpNP-TUP1	0.095 ± 0.001	0.079 ± 0.002**	1.20	4.0 ± 0.2	3.16 ± 0.06**	1.27
Sc tup1⁻ + YEpNP	0.077 ± 0.005	0.069 ± 0.001	1.10	3.57 ± 0.07	3.5 ± 0.2	1.02

Strains were cultured at 22 °C in SD medium supplemented only with 2% sucrose (suc) or that in addition to 0.02% 2-deoxy-d-glucose (suc-2DG). Each µ value corresponds to the average of three independents cultures and errors to the standard deviation. OD_max: maximum value of OD₆₀₀ reached during the time period studied
** p < 0.01, Student’s t test (compared to the control condition)

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