Fig. 4

Heterologous complementation of the X. dendrorhous CYC8 and TUP1 genes in S. cerevisiae. a Growth curves of strains S288c, Sccyc8 ⁻+YEpNP-CYC8, Sccyc8 ⁻+YEpNP, Sctup1 ⁻+YEpNP-TUP1 and Sctup1 ⁻+YEpNP cultured in SD-2% glucose at 30 °C. Significant differences between µ values are represented by different letters (a–d) based on ANOVA with Tukey’s post-test (α = 0.05; p ≤ 0.05). b Invertase activity in samples taken from cultures at the early exponential growth phase. The strains were cultured at 22 °C in SD-2% glucose (repressive conditions) or SD-2% sucrose (non-repressive conditions). YDW yeast dry weight. The data represent the average of three independent cultures with their respective standard deviations (error bars). **p < 0.01, Student’s t test