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Table 3 Results of the microtiter-plate screening of the biosensors strains on glucose, xylose and a co-culture thereof, given in terms of FI fold induction

From: Real-time monitoring of the sugar sensing in Saccharomyces cerevisiae indicates endogenous mechanisms for xylose signaling

Strain

Glucose 5 g/La

Xylose 50 g/L + Gluc. 5 g/L

Xylose 50 g/Lb

3 h

6 h

3 h

6 h

3 h

6 h

TMB3711 (No GFP)

1.00 ± 0.010

0.92 ± 0.056

1.04 ± 0.010*

1.02 ± 0.041 *

0.79 ± 0.017

0.78 ± 0.023

TMB3712 (HXT1p)

1.21 ± 0.016

1.23 ± 0.013

1.48 ± 0.011*

1.70 ± 0.011 *

0.83 ± 0.015

0.84 ± 0.010

TMB3713 (HXT2p)

1.10 ± 0.264

1.34 ± 0.11

1.37 ± 0.26

2.01 ± 0.55*

0.89 ± 0.039

0.89 ± 0.046

TMB3714 (HXT4p)

1.77 ± 0.058

2.47 ± 0.22

2.15 ± 0.31*

3.45 ± 0.62*

1.08 ± 0.25

1.31 ± 0.51

TMB3715 (SUC2p)

0.98 ± 0.15

3.51 ± 0.75

1.13 ± 0.10

5.15 ± 0.62*

0.95 ± 0.12

1.06 ± 0.21

TMB3716 (CAT8p)

0.98 ± 0.002

1.14 ± 0.039

1.08 ± 0.037*

1.13 ± 0.037

0.82 ± 0.003

0.84 ± 0.018

TMB3717 (TPS1p)

0.74 ± 0.051

1.23 ± 0.075

0.70 ± 0.048

0.77 ± 0.047*

0.92 ± 0.006

0.91 ± 0.029

TMB3718 (TPS2p)

0.85 ± 0.026

1.61 ± 0.084

0.95 ± 0.076*

1.05 ± 0.032*

0.85 ± 0.012

0.85 ± 0.026

TMB3719 (TEF4p)

1.35 ± 0.013

1.46 ± 0.010

1.28 ± 0.018*

1.42 ± 0.0032

0.87 ± 0.012

0.96 ± 0.010

  1. The FI signal was normalized to the corresponding 0 h signal of the given condition and strain. A value of 1 corresponds to repression (i.e. no fold change since time 0 h). A one-way ANOVA with a multiple comparison test was performed to statistically compare the results from the different conditions
  2. * Significantly different from the glucose 5 g/L fold change from the same hour (one-way ANOVA with a multiple comparison test; p ≤ 0.05)
  3. aGlucose 5 g/L was significantly different from the xylose 50 g/L in all strains and times except for 3 h for TMB3713, TMB3715, TMB3718 and 6 h for TMB3713
  4. bXylose 50 g/L was significantly different from the xylose-glucose co-culture in all strains and times except for TMB3715 at 3 h