Table 1 Yeast strains and plasmids used in the present study
Strain | Genotype | Reference |
|---|---|---|
W303-1A | MATa trp1-1 leu2-3,112 his3-11 ade2-1 ura3-1 can1-100 | [54]; ATCC® 208352 |
TMB3700 | W303-1A TRP1 HIS3, ura3::M3499 (ADE2) | This study |
TMB3711 | TMB3700; can1::YIp211; SPB1/PBN1::YIp128 | This study |
TMB3712 | TMB3700, can1::YIpGFP-Hxt1p; SPB1/PBN1::YIp128 | This study |
TMB3713 | TMB3700, can1::YIpGFP-Hxt2p; SPB1/PBN1::YIp128 | This study |
TMB3714 | TMB3700, can1::YIpGFP-Hxt4p; SPB1/PBN1::YIp128 | This study |
TMB3715 | TMB3700, can1::YIpGFP-Suc2p; SPB1/PBN1::YIp128 | This study |
TMB3716 | TMB3700, can1::YIpGFP-Cat8p; SPB1/PBN1::YIp128 | This study |
TMB3717 | TMB3700, can1::YIpGFP-Tps1p; SPB1/PBN1::YIp128 | This study |
TMB3718 | TMB3700, can1::YIpGFP-Tps2p; SPB1/PBN1::YIp128 | This study |
TMB3719 | TMB3700, can1::YIpGFP-Tef4p; SPB1/PBN1::YIp128 | This study |
CEN.PK 113-7D | MATα, MAL2-8 C , SUC2 | [76]; EUROSCARF collection |
Plasmid | Relevant genotype | Reference |
|---|---|---|
YIplac128 | AmpR; LEU2 | [50] |
YIplac211 | AmpR; URA3 | [50] |
YEpJK01 | YEplacHXT; URA3; GPD2p-yEGFP3-PGK1t | [33] |
YIpGFP | YIplac211: yEGFP3-PGK1t | This study |
YIpGFP-HXT1p | YIpGFP:HXT1p-yEGFP3-PGK1t | This study |
YIpGFP-HXT2p | YIpGFP:HXT2p-yEGFP3-PGK1t | This study |
YIpGFP-HXT4p | YIpGFP:HXT4p-yEGFP3-PGK1t | This study |
YIpGFP-SUC2p | YIpGFP:SUC2p-yEGFP3-PGK1t | This study |
YIpGFP-CAT8p | YIpGFP:CAT8p-yEGFP3-PGK1t | This study |
YIpGFP-TPS1p | YIpGFP:TPS1p-yEGFP3-PGK1t | This study |
YIpGFP-TPS2p | YIpGFP:TPS2p-yEGFP3-PGK1t | This study |
YIpGFP-TEF4p | YIpGFP:TEF4p-yEGFP3-PGK1t | This study |
M3499 | AmpR; ura3::ADE2 | [52] |